The Thermodynamics of Protein Separations

9510420 Lenhoff这个提案是关于继续发展定量热力学基础的研究，用于分析和设计蛋白质分离，可与化学过程工业中常规应用的常规分离相比较。这将涉及以下研究：(1)基于已知蛋白质结构的分子理论，预测溶液中蛋白质的关键热力学性质，以及基于未知结构的关键实验生物物理特性；(2)利用相行为和物理性质的描述，开发合理的、基于热力学的方法，预测重要过程的分离性能，包括沉淀和两相水萃取。＊＊＊

9510420 Lenhoff This proposal is on research to continue to develop a quantitative thermodynamic basis for the analysis and design of protein separations, comparable to that which is routinely applied to conventional separations in the chemical process industries. This will involve research on: (1) molecular theories to predict key thermodynamic properties of proteins in solution, based on protein structure, when it is known, and on key experimental biophysical characteristics, when the structure is not known, and (2) use of the descriptions of phase behavior and physical properties to develop rational, thermodynamically- based approaches to predict separation performance for important processes, including precipitation and two-phase aqueous extraction. ***