Regulation of Membrane Lipid Hydrolysis in Lipid-based Signaling of Plants

9511623王脂为基础的信号途径通过十八烷类途径，导致茉莉酸等调节剂的形成，在植物的生理过程中发挥着重要的作用。茉莉酸和相关的氧化脂质被证明是内源信号，激活防御基因的表达，调节生长发育。α-亚麻酸已被证明是合成这些调节剂的前体。然而，人们对α-亚麻酸在十八烷类信号通路中的脂解释放知之甚少。在信号传递过程中，哪种脂肪水解酶被激活？哪些甘油脂是被水解物？水解度是如何调节的？这些问题很重要，因为获得脂肪酸前体是信号转导过程中一系列事件的早期步骤，并可能控制信号通路。因此，这项研究的目的是调查脂解酶在十八烷类信号通路中的参与、性质和脱酰化的调节。刺激诱导的脂代谢和α-亚麻酸的释放将被研究在蓖麻叶中对伤害的反应。选择伤害是因为它是一种很好的刺激，可以诱导茉莉酸信号系统。这项拟议工作的完成将填补我们对膜脂水解酶在植物生长和环境响应中的细胞作用的知识的一个关键空白。

9511623 Wang Lipid-based signaling via the octadecanoid pathway, which leads to the formation of regulators such as jasmonic acid, plays an important role in plant physiological processes. Jasmonate and related oxygenated lipids have been shown to act as endogenous signals activating defense gene expression and regulating growth and development. Alph-linolenic acid has been shown to be the precursor for the synthesis of these regulators. However, very little is known about lipolytic release of alpha-linolenic acid in the octadecanoid signaling pathway. Which lipid hydrolyzing enzyme is activated in the signaling process? Which glycerolipids are hydrolyzed? How is the hydrolysis regulated? These questions are important because making available the fatty acid precursor is an early step in the sequence of events in signal transduction and may control the signaling pathway. The goal of this research is, therefore, to investigate the involvement, nature, and regulation of deacylation by lipolytic enzymes in the octadecanoid signaling pathway. Stimulus-induced lipid turnover and release of alpha-linolenic acid will be investigated in castor bean leaves in response to wounding. Wounding is chosen because it is a well characterized stimulus that induces the jasmonate signaling system. Completion of the proposed work will fill a critical gap in our knowledge of the cellular roles of membrane lipid-hydrolyzing enzymes in plant growth and environmental responsiveness.