Workshop on Site-specific Recombination and Transposition, September 22-27, 1996, Woods Hole, MA

9600629 Kleckner和Mizuuchi本研讨会的目的是聚集整个科学家社区进行讨论，他们正在“积极机械”的水平上研究特定位点重组和转位的生化机制。这一组相互关联的反应位于许多非常重要的生物学过程的核心：逆转录病毒的整合、抗体多样性的产生、抗生素耐药基因的传播和染色体的重排。对这些反应的分析需要了解和应用许多科学学科，包括蛋白质生物化学、生化动力学、化学、DNA拓扑学和遗传学，相关社区内的科学分析和专业知识水平非常高。该科学计划包括逆转录病毒整合、原核转座子、整合酶和解旋酶家族的位点特异性重组酶、免疫球蛋白重排和与这些反应有关的辅助宿主因子的研究。此外，该计划还包括研究其他类型的蛋白质/DNA反应中的化学催化作用，以便该领域的观察可以放在更广泛的背景下进行。最后，会议包括几个关于“模型系统”研究的讲座，这些研究在领域之外，但与所提出的问题的类型和/或用于解决MF感兴趣的问题的方法的类型直接相关。会议将限制在135人参加。这一数字足以包括在这一领域工作卓有成效的所有主要研究人员以及许多博士后研究员和一些研究生。基本上，每个与会者要么发言，要么展示海报。这次会议已成为这一领域的知识协调中心，在正式和非正式两级与会者之间的交流程度上是独一无二的。***

9600629 Kleckner and Mizuuchi The purpose of this Workshop is to assemble for discusqions essentially the entire community of scientists who are working on the biochemical mechanisms of site-specific recombination and transposition at an "aggressively mechanistic" level. This interrelated group of reactions lie at the heart of a number of very important biological processes: integration of retroviruses, generation of antibody diversity, transmission of antibiotic resistance genes and rearrangements of chromosomes. The analysis of these reactions requires understanding and applications of many scientific disciplines including protein biochemistry, biochemical kinetics, chemistry, DNA topology and genetics, and the level of scientific analysis and expertise within the relevant community is very high. The scientific program includes studies of retroviral integration, prokaryotic transposons, site-specific recombinases of the integrase and resolvase families, immunoglobulin rearrangements and accessory host factors as they pertain to these reactions. In addition, the program includes studies of chemical catalysis in other types of protein/DNA reactions so that observations in the field can be placed in a broader context. Finally, the meeting includes several talks on "model systems" studies which are outside the field but are outside the field but are directly relevant with respect to the types of questions being asked and/or the types of methods being used to address questions mf interest. The meeting will be limited to 135 participants. This number is sufficient to include all of the principal investigators who are working productively in this area as well as many post-doctoral fellows and some graduate students. Essentially every attendee either speaks or presents a poster. This meeting has become the intellectual focal point for this field and is unique in the degree of interchange amongst participants at both formal and informal levels. ***