CAREER: Biological and Molecular Studies of Trypanosomal Ribonucleases H

9600920坎贝尔本职业计划的研究部分涉及RNase H，广泛分布于从逆转录病毒到人类的系统中的酶。 它们特异性地降解RNA：DNA双链体杂交体的RNA链，这是DNA复制中的重要功能。 RNase H的分布进一步表明，它们在所有生物系统中处于调节DNA复制和核酸代谢关键决定的中心位置。 坎贝尔博士将启动研究，以确定RNA酶H在真核DNA复制和细胞生长中发挥的作用，使用原生动物布氏锥虫。T.将建立布鲁塞;将确定表达和纯化的重组酶的活性模式;并鉴定导致催化改变的酶的突变。 该教育计划包括对最近引入布朗大学本科课程的一门新课程进行创新性改革，并实施一项指导和激发少数民族学生学习和科学兴趣的计划。 RNase H是一种特异性降解DNA：RNA杂交体的RNA链的酶（H代表杂交体）。RNase家族分布广泛，存在于从逆转录病毒到人类的系统中。它们在控制DNA复制中起作用，因此对逆转录病毒以及真核生物的生命周期至关重要。 在这个项目中，RNase H在一个复杂的生物体中的作用，原生动物布氏锥虫，将被研究，以了解更多关于它们在细胞中的功能。教育部分为现代生物学基本概念的新课程增加了创新性的变化，以及刺激代表性不足的学生追求学术生涯的计划。 ***

9600920 Campbell The research portion of this CAREER proposal concerns RNases H, widely distributed enzymes found in systems ranging from retroviruses to humans. They specifically degrade the RNA strands of RNA:DNA duplex hybrids, an important function in DNA replication. The distribution of RNases H further suggests that they are centrally positioned to regulate key decisions in DNA replication and nucleic acid metabolism in all biological systems. Dr. Campbell will initiate studies to establish the roles played by RNases H in eukaryotic DNA replication and cell growth using the protozoan Trypanosoma brucei. Expression patterns of the major RNase H, TbbRNHI, of T. Brucei will be established; activity patterns of the expressed and purified recombinant enzyme will be defined; and mutations that result in catalytically altered enzyme will be identified. The educational plan consists of adding innovative changes to a new course recently introduced into the Brown undergraduate curriculum and implementing a program to mentor and ignite under-represented minority student learning and scientific interest. RNase H is an enzyme that specifically degrades the RNA strand of DNA:RNA hybrids (H for hybrid). The RNase family is widely distributed, found in systems ranging from retroviruses to humans. They are function in controlling the replication of DNA, and thus are critical to the life cycles of retroviruses as well as eukaryotic organisms. In this project, the role of RNase H in a complex organism, the protozoan Trypanosoma brucei, will be studied in an effort to learn more about their function in cells. The education component adds innovative changes to a new course on fundamental concepts in modern biology, as well as a program to stimulate under-represented students to pursue academic careers. ***