Identification of Host Factors Required for Replication of Geminivirus DNA

9601893罗伯逊番茄金花叶病毒（TGMV）在细胞核内复制，激活了细胞周期。增殖细胞核抗原（PCNA）是DNA聚合酶γ的辅助因子，在感染细胞中与TGMV共定位，但在健康植物的可比分化细胞中不存在。PCNA是与双病毒感染相关的宿主蛋白的第一个例子。这项工作的具体目标是：1)确定TGMV感染是否引起细胞周期调节蛋白的积累；2)确定病毒复制蛋白AL1是否引起其他宿主蛋白的表达增加；3)确定TGMV DNA是否优先复制或染色体DNA是否也被复制。这项研究的结果将对宿主植物中病毒发病和细胞周期调节的机制和可能的联系提供重要的见解。加强对这种相互作用的了解，可能会开发出控制植物病毒性疾病的新方法。＊＊＊

9601893 Robertson Tomato golden mosaic virus (TGMV) replicates in nuclei of cells which have excited the cell cycle. Proliferating cell nuclear antigen(PCNA), an accessory factor for DNA polymerase gamma, was co-localized with TGMV in infected cells but was absent in comparable, differentiated cells of healthy plants. PCNA is the first example of a host protein associated with geminivirus infections. The specific goals of this work are: 1) To determine if TGMV infection causes the accumulation of cell cycle regulatory proteins 2) To determine if the viral replication protein, AL1, causes an increase in the expression of other host proteins and 3)To determine if TGMV DNA is preferentially replicated or if chromosomal DNA is also replicated. %%% The results of this investigation should lead to important insights into the mechanisms and possible linkage of viral pathogenesis and cell cycle regulation in the host plant. New approaches for the control of viral plant diseases may be developed from the enhanced understanding of such interactions. ***