CRI: In Vivo Neural Gene Imaging Using a Novel Radiation Detector

IBN：9602865 PI：Saha 研究大脑发育的神经科学家对此非常感兴趣 并且其功能是能够跟随由生物体的每个细胞中储存的遗传密码指导的基因表达。 目前，如果想了解遗传密码的哪一部分 在实验室老鼠的胚胎发育中使用， 在各种药物治疗中被激活， 必须牺牲有机体，然后使用分子生物学技术来研究被激活的基因。 这个方法本质上只需要一个 基因发生过程的“快照”。 然后，一个人需要开始与另一个有机体，并希望采取另一个'快照'在 正是时候 这个拟议中的项目将使用新的辐射成像方法，已开发的粒子物理学研究一起 利用医学成像技术， 对生物体内的基因激活模式进行成像，即， 而不必牺牲胚胎。 这将使我们能够产生一个连续的帐户的遗传过程发生 在神经系统中。 我们的最终目标是制造一个探测器系统 能够在发育过程中对基因进行成像， 实验药理学治疗--一个最终将被应用于更好地理解和治疗人类神经系统疾病的系统。

IBN: 9602865 PI: Saha It is of great interest to neuroscientists studying brain development and function to be able to follow gene expression which is directed by the genetic code stored in each cell of an organism. Presently if one wants to understand what part of the genetic code is being used in a developing embryo of a laboratory mouse or what genes are activated in response to varous pharmacological treatments, one must sacrifice the organism and then use molecular biology techniques to study the genes being activated. This method is essentially taking only a 'snapshot' of the genetic processes occurring. One then needs to begin anew with another organism and hope to take another 'snapshot' at the right time. This proposed project will use new radiation imaging methods that have been developed for particle physics research together with techniques used in medical imaging to allow the neuroscientist to image the gene activation patterns in an organism in vivo, that is, without having to sacrifice the embryo. This will enable us to produce a continuous account of the genetic processes occurring within the nervous system. Our ultimate goal is to produce a detector system capable of imaging genes during development and in response to various experimental pharmacological treatments -- a system which will eventually be applied to better understanding and treating human neurological disease.