Desorption Ionization Studies Using Fourier Transform Mass Spectrometry

该项目得到分析和表面化学计划的支持，目标是建造和表征一台使用离子回旋共振和离子迁移率光谱的串联仪器和一台飞行时间质谱仪(ICR/IMS/TOF MS)，用于测定生物大分子的体积。ICR将选择特定的质荷离子，IMS将按体积对这些特定的质荷离子进行分类，TOF MS将检测这些离子。同质异构体离子体积的测量将提供这些生物大分子不同结构形式的部分组成信息。在这项工作中，罗素教授和他在德克萨斯农工大学化学系的学生将构建一种新的仪器，该仪器将与基质辅助激光解吸电离(MALDI)MS一起使用，以探索相参数如何影响分子构象。这个项目的目标是建立和表征一种新的串联质谱仪，可以用来确定生物大分子的结构。罗素教授和他在德克萨斯农工大学的学生将使用离子回旋共振原理、离子迁移率光谱仪和飞行时间质谱仪来建造这台仪器。然后，该仪器将耦合到基质辅助激光解吸电离源，以便从固体基质引入气相的大生物分子然后可以根据它们的结构进行分类和表征。其目的是确定固体基质对生物分子结构的影响。这一新仪器将在生物技术和化学科学的许多领域发挥作用。

This project, supported in the Analytical and Surface Chemistry Program, targets the construction and characterization of a tandem instrument using ion cyclotron resonance with ion mobility spectrometry and a time-of-flight mass spectrometer (ICR/IMS/TOF MS) for the determination of the volumes of large biological molecules. The ICR will select particular mass-to-charge ions, the IMS will sort these particular mass-to-charge ions by volume, and the TOF MS will detect these ions. Measurement of ion volumes for isomass ions will provide fractional composition information about different structural forms of these large biological molecules. In this work Professor Russell and his students of the Department of Chemistry at Texas A & M University will construct a new instrument that will be used in conjunction with matrix assisted laser desorption ionization (MALDI) MS to probe how phase parameters influence molecular conformation. The goal of this project is to build and characterize a new tandem mass spectrometer that can be used to determine the structure of large biological molecules. Professor Russell and his students at Texas A & M University will construct this instrument using principles of ion cyclotron resonance, an ion mobility spectrometer, and a time-of-flight mass spectrometer. This instrument will then be coupled to a matrix assisted laser desorption ionization source so that large biological molecules introduced into the gas phase from the solid matrix can be then sorted and characterized according to their structure. The aim is to determine the affect of the solid matrix upon the structure of the biological molecules. This new instrument will be useful in a number of areas in biotechnology and in the chemical sciences.