In vitro Reconstruction of Fungal Chromosomes

全染色体的物理图谱在遗传学的一些基本突破中发挥了重要作用。完成了模型遗传系统的高分辨率物理图。粗神经孢子虫的研究可能为真菌分子进化遗传学的研究开辟新的途径。在完成初步的29千碱基（kb）分辨率物理地图的31百万碱基的细粒曲霉基因组，一个惊人的观察作出。重复序列沿每条染色体的分布呈交替带状。这一发现对基因组的发育、功能组织、染色体结构和功能、重组和基因组进化具有重要意义。在这个项目中，生物学上的问题是这种重复序列的条带模式是否在另一种丝状真菌中发现，n.g assa基因组将使用nsf资助的机器人工作站完成。与nsf资助的cDNA测序项目合作，dna的分布将与重复序列的分布相关。在丝状真菌、细粒曲霉和粗神经孢子菌中，关于DNA的结构和功能有了许多重要的发现。它们的真菌亲戚是我们日常生活的一部分，是植物和动物疾病、食物腐败以及工业酶和化学品制造的原因。这些真菌的亲戚涉及从酱油、奶酪和其他乳制品的制造到青霉素和各种纺织品的制造等方方面面。在这个项目中，新的基因组技术将被引入真菌遗传学社区，以改变遗传学是如何完成的。新的基因组技术将绘制出这些生物的整个遗传蓝图，并使这一遗传蓝图能够被理解和操纵。该项目的应用可能会对遗传学以及农业和工业的应用产生影响。

Arnold 9630910 Physical maps of whole chromosomes have played an essential role in a number of fundamental breakthroughs in genetics. Completion of a high resolution physical map of the model genetic system. Neurospora crassa, is likely to open up new avenues of research on the molecular and evolutionary genetics of fungi. In completing a preliminary 29 kilobase (kb) resolution physical map of the 31 megabase Aspergillus nidulans genome, a striking observation was made. There is an alternating banding pattern in the distribution of repeated sequences along each chromosome. This observation has implications for development, functional organization of genomes, chromosome structure and function, recombination, and genome evolution. In this project the biological question is whether or not this banding pattern in repeated sequences is found in another filamentous fungus, N.crassa genome will be completed using an NSF-funded robotics workstation. The distribution of cDNAs will be correlated with the distribution of repeated sequences in collaboration with an NSF-funded cDNA sequencing project. A number of important discoveries about the structure and function of DNA have been made in the filamentous fungi, Aspergillus nidulans and Neurospora crassa. Their fungal relatives are a daily part of our lives as causes of plant and animal disease, food spoilage, and the manufacture of industrial enzymes and chemicals. Relatives of these fungi are involved in everything from the making of soy sauce, cheeses, and other dairy products to the manufacturing of penicillin and various textiles. In this project new genome technologies will be introduced into the fungal genetics community to transform how genetics is done. The new genome technologies will chart the entire genetic blueprint of these organisms, and allow this genetic blueprint to be understood and manipulated. The applications of this project are likely to have an impact on genetics in general as well as applications in agriculture, and industry.