Molecular Structure and Function of the Arabidopsis RPM1 Disease Resistance Gene

9724075 Dangl对导致植物抗病的分子事件的详细理解是一个有趣的基础研究挑战。病原菌基因座的等位基因与相应植物抗病基因座的等位基因之间的特定相互作用往往决定了对病害反应的遗传控制。最近在这一领域的突破包括第一个抗性基因的分子鉴定，包括RPM1，这是拟议工作的重点。由RPM1编码的蛋白(S)以一种未知的方式识别病原体衍生的因子。RPM1蛋白的参与触发了信号转导和防御反应。一个重要的结果是，RPM1编码了一种双重特异性，通过诱导对另一种无关病原体功能的作用产生抵抗。这项拟议的工作将解决RPM1蛋白的结构-功能关系、其亚细胞定位以及与RPM1相互作用的蛋白质的数量和性质。

9724075 Dangl A detailed understanding of the molecular events leading to plant disease resistance presents an interesting basic research challenge. Genetic control of disease reactions is often determined by specific interactions between alleles of a pathogen gene locus and an allele of the corresponding plant disease resistance locus. Recent breakthroughs in this field include the molecular identification of the first resistance genes, including RPM1 which is the focus of the proposed work. The protein(s) encoded by Rpm1 recognize pathogen-derived factors in an as yet unknown manner. Engagement of the RPM1 protein triggers signal transduction and defense responses. One important result is that RPM1 encodes a dual specificity , by eliciting resistance to the action of another, unrelated pathogen function. The proposed work will address structure-function relationships of the RPM1 protein, its subcellular localization and the number and nature of proteins with which RPM1 interacts.