Acquisition of Major Multiuser Research Instrumentation for the Study of Plant, Fungal and Insect Genomics

这项建议的主要目标是获得主要仪器，用于分析复杂的植物、真菌和昆虫基因组的研究和培训。仪器包括：5台自动化DNA测序仪，1台用于制备DNA测序模板的机器人，9台用于处理序列和物理映射数据的计算机工作站，1台用于开发半自动化程序的机器人系统，该程序用于高通量筛选数千份DNA样品，用于文库筛选和植物育种应用，12台用于维护大型插入B AC文库的超低冷冻机，6个杂交培养箱和一个多功能成像系统，用于快速筛选和分析BAC文库。 所要求的仪器将与现有的机器人设备，正在进行的研究，服务和教学计划相结合，以建立一个最先进的基因组中心在克莱姆森大学（CU）。这个新的CU基因组中心的功能是：1）构建和维护高质量的BAC文库; 2）生产和分发高密度杂交滤膜和DNA池，用于文库筛选和BAC文库和克隆的分发; 3）农业重要基因的定位克隆; 4）用BAC克隆对整个染色体和靶基因组区域进行物理作图; 5）重要BAC重叠群和基因组的基因组测序; 6）应用开发;以及7）本科生、研究生和博士后培训。 有三项主要活动将通过为这项提议提供资金而得到极大的加强。首先，MRI资金将确保CU基因组中心能够作为重要植物，真菌和昆虫基因组的大型插入BAC文库的构建，维护和分发的公共资源。这样一个集中的资源将使研究界能够方便地获得用于定位克隆、物理绘图和基因组测序的主要工具。第二，MRI的资助将使我们能够执行一些试点项目，这些项目的目的是对基因组的特定基因组区域进行物理绘图和测序，这些区域正被作为深入研究的目标。这些项目的成功完成将使CU基因组中心能够有效地竞争大量的联邦支持，以对拟南芥、水稻和粗糙脉孢菌等基因组的主要部分进行测序。最后，MRI资金将大大支持和加强基因组学的本科生，研究生和博士后培训。通过使用最先进的仪器，CU基因组中心将吸引高素质的学生和学者，推动几个生命科学项目进入国家排名，例如遗传学的跨学科项目。 CU基因组中心，如上所述，将是一个国际公认的设施，反过来将产生世界一流的科学服务于农业基因组社区在整个。

The primary objective of this proposal is to acquire major instrumentation for research and training in the analyses of complex plant, fungal and insect genomes. The instrumentation includes: 5 automated DNA sequencers, a robot for preparing DNA sequencing templates, 9 computer workstations for processing sequence and physical mapping data, a robotics system for the development of semi-automated procedures for high through-put screening of thousands of DNA samples for library screening and plant breeding applications, 12 ultra-low freezers for maintenance of large insert B AC libraries, a bank of 6 hybridization incubators and a versatile imaging system for rapid screening and analysis of BAC libraries. The requested instrumentation will be integrated with existing robotics equipment, and ongoing research, service and teaching programs to establish a state-of-the-art Genome Center at Clemson University (CU). The functions of this new CU Genome Center are: 1 ) construction and maintenance of high quality BAC libraries; 2) production and distribution of high-density hybridization filters and DNA pools for library screening and distribution of BAC libraries and clones; 3) positional cloning of agriculturally important genes; 4) physical mapping of whole chromosomes and targeted genomic regions with BAC clones; 5) genome sequencing of important BAC contigs and genomes; 6) application development; and 7) undergraduate, graduate and postdoctoral training. There are three primary activities that would be greatly enhanced by funding this proposal. First, MRI funding would ensure that the CU Genome Center will be capable of serving as a public resource for the construction, maintenance and distribution of large insert BAC libraries of important plant, fungal and insect genomes. Such a centralized resource would provide the research community convenient access to a major tool that is used for positional cloning, physical mapping and genome sequencing. Secondly, MRI funding would allow us to perf orm pilot projects aimed at physical mapping and sequencing of specific genomic regions of genomes that are being targeted for intensive investigation. Successful completion of these projects will put the CU Genome Center in a position to compete effectively for substantial federal support to sequence major portions of genomes such as Arabidopsis, Rice and Neurospora crassa. Lastly, MRI funding will substantially support and enhance undergraduate, graduate, and postdoctoral training in genomics. With access to state-of-the-art instrumentation, the CU-Genome Center will attract students and scholars of high caliber propelling several life sciences programs to national ranking, such as the interdisciplinary program in Genetics. The CU Genome Center, as envisioned above, will be an internationally recognized facility that in turn will produce world class science serving the agricultural genome community at large.