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Hydrophobic Displacement Chromatography of Proteins

Hydrophobic Displacement Chromatography of Proteins
蛋白质的疏水置换色谱
批准号:
9810794
负责人:
Steven Cramer
金额:
$23.68万
依托单位国家:
美国
项目类别:
Continuing Grant
财政年份:
1998
资助国家:
美国
项目状态:
已结题
起止时间:
1998-12-01 至 2002-08-31

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中文摘要
翻译
这位研究人员最近发现,低分子排代剂可用于促进疏水相互作用(HIC)和反相(RPLC)色谱系统。这一提议将试图在一个同时具有高通量和选择性的系统中获得置换和吸附机制的好处。将为HIC和RPLC的一系列蛋白质确定低分子代替物,并为这些系统开发分离等温线。操作问题,如驱替剂的移除和回收,柱再生,以及拆分能力的优化,将被研究。最后,将通过从复杂的生物混合物中分离蛋白质来测试混合方法。
英文摘要
This investigator has recently found that low molecular weight displacers can be used to facilitate hydrophobic interaction (HIC) and reversed phase (RPLC) chromatographic systems. This proposal will attempt to capture the benefits of the displacement and adsorption mechanisms in one system capable of both high throughput and selectivity. Low molecular weight displacers will be identified for a range of proteins for both HIC and RPLC, and separation isotherms developed for these systems. Operational problems such as displacer removal and recovery, column regeneration, and optimization of the resolving power will be investigated. Finally, the hybrid method will be tested by separating proteins from complex biological mixtures.
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Preferred binding regions for highly selective and orthogonal multimodal protein separations
  • 批准号:
    1704745
  • 项目类别:
    Standard Grant
  • 资助金额:
    $32.65万
  • 财政年份:
    2017
  • 负责人:
    Steven Cramer
  • 依托单位:
Travel Grant for Eighth Mediterranean Chemical Engineering Conference to be held in Israel in 2014
  • 批准号:
    1248189
  • 项目类别:
    Standard Grant
  • 资助金额:
    $4.8万
  • 财政年份:
    2012
  • 负责人:
    Steven Cramer
  • 依托单位:
Molecular Engineering of Multiple Weak Interactions for High Selectivity Bioseparations
  • 批准号:
    1160039
  • 项目类别:
    Standard Grant
  • 资助金额:
    $34.0万
  • 财政年份:
    2012
  • 负责人:
    Steven Cramer
  • 依托单位:
Travel Grant for Seventh Mediterranean Chemical Engineering Conference, Greece
  • 批准号:
    1137546
  • 项目类别:
    Standard Grant
  • 资助金额:
    $4.8万
  • 财政年份:
    2011
  • 负责人:
    Steven Cramer
  • 依托单位:
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