SGER: MALDI MS Analysis of Roughness Effects on Protein Binding to Pulsed Plasma Modified Polymer Surfaces

本研究的目的是研究一种新的分析方法来研究表面-生物分子相互作用的动力学。这种方法是矩阵辅助激光解吸电离（MALDI）质谱法（MS）。该技术可以在小于单层覆盖的情况下检测生物分子的表面吸附。要研究的问题集中在表面粗糙度对衰减MALDI MS离子信号的影响上。从本质上讲，已经观察到一种反比关系，即给定一定量的生物分子应用于给定的聚合物表面，生物分子MALDI离子信号强度随着表面与生物分子结合亲和力的降低而增加。这些研究，连同对各种表面性质变化对生物分子吸附的影响的研究和对生物分子吸附的时间依赖性的研究，有望为影响表面-生物分子相互作用的基本过程提供新的见解。＊＊＊

9812708 Kinsel The goal of this proposed research is to study a new analytical approach to examine the dynamics of surface- biomolecule interactions. This approach is that of Matrix Assisted Laser Desorption Ionization (MALDI) mass spectrometry (MS). This technique could enable the examination of biomolecule surface adsorption at less than monolayer coverage. The problem to be examined centers on the effect of surface roughness in attenuating MALDI MS ion signals. Essentially, an inverse relationship has been observed in which, given a constant amount of biomolecule applied to a given polymer surface, the biomolecule MALDI ion signal increases in intensity as the surface-biomolecule binding affinity decreases. These studies, together with studies of the effect of changes in various surface properties on biomolecule adsorption and studies of the time dependence of biomolecule adsorption, promise to provide new insights into the fundamental processes which influence surface-biomolecule interactions. ***