XYZ on a Chip: Electroactive Monolayers for Integrating Circuits with Mammalian Cells

9980850Mrksich这个工程微系统的目标：“XYZ”芯片项目将开发一个基本的方法，集成的功能，细胞和材料组件的混合设备。链烷硫醇盐在金上的自组装单分子膜将被用作控制细胞和基底之间相互作用的模型基底。该计划将开发电活性单分子层，可以调节，在真实的时间，与附着细胞的表面受体相互作用的固定化配体的活性。该策略是基于电活性基团的设计，当电位施加到下面的金时，电活性基团进行氧化或还原。当这些电活性基团被引入到固定在单层上的配体的结构中时，它们可用于影响配体活性的变化。该计划将提供三类电活性单层，可用于控制附着细胞的行为。这些包括选择性释放固定化配体的单层、开启固定化配体的活性的单层和可逆地调节固定化配体的活性的单层。一个相关的努力将开发电活性配体，可以报告细胞受体的结合，从而将生物信号转化为电输出。由于这类单层被设计成有效地将电信号转换成生物信号，因此它代表了集成电路成熟技术的附加能力。这里开发的策略可能是重要的，以设计一个广泛的混合设备，结合联合收割机细胞和材料组件用于诊断，组织工程，留置传感器，神经计算，和BioMEMS。

9980850MrksichThe goal of this Engineering Microsystems: "XYZ" on a Chip project will develop a fundamental approach for integrating the functions of cellular and materials components in hybrid devices. Self-assembled monolayers of alkanethiolates on gold that present peptide ligands will be used as model substrates that control the interactions between cells and the substrate. The program will develop electroactive monolayers that can modulate, in real time, the activity of immobilized ligands that interact with the surface receptors of attached cells. The strategy is based on the design of electroactive groups that undergo oxidation or reduction when electrical potentials are applied to the underlying gold. When these electroactive groups are incorporated into the structures of ligands immobilized to the monolayers, they can be used to effect changes in the activity of the ligands. This program will deliver three classes of electroactive monolayers that can be used to control the behaviors of attached cells. These include monolayers that selectively release immobilized ligands, monolayers that turn on the activity of immobilized ligands, and monolayers that reversibly modulate the activity of immobilized ligands. A related effort will develop electroactive ligands that can report on the binding of cellular receptors, and hence transduce biological signals into electrical outputs. Because this class of monolayers is designed to efficiently convert electrical signals into biological signals, it represents an add-on capability to the mature technology of integrated circuits. The strategies developed here may be important to designing a broad range of hybrid devices that combine cellular and materials components for use in diagnostics, tissue engineering, indwelling sensors, neural computation, and BioMEMS.