Quantitative Data Analysis and Graphics Facility: Storm 860 and Alpha Imagers

0070321詹姆斯·D·福尔尼普渡研究基金会定量数据分析和图形设施：STORM 860和阿尔法成像仪该奖项将资助开发一个部门间的生化和分子生物学数据记录和量化设施。该设施将提供设备和软件，将大多数凝胶电泳实验得出的数据数字化，并允许对结果数据进行分析。可以分析的程序和技术包括：DNA和RNA测序和结构分析，基于聚合酶链式反应的分析，包括定量PCR和RFLP分析，定量Northern和Western印迹，Southern杂交，研究和教学实验室的非放射性检测，菌落计数和酶反应动力学分析。目的是通过使设施尽可能灵活和方便地最大化使用。要购买的设备包括两台成像仪，能够量化使用自动放射成像、荧光染色或标签、可见染色或标签或简单地使用白光成像的数据。这两个组件是Storm 860成像仪(分子动力学)和AlphaImager(AlphaInnotech)。将购买足够的计算设备进行图像和数据分析。该设施将安装在位于中心位置的生物化学大楼的一楼，这将为几个部门的研究人员提供便利。该设施将用于本科生和研究生水平的量化和分析从生物检测收集的数据方面的培训。

0070321James D. ForneyPurdue Research FoundationQuantitative Data Analysis and Graphics Facility: Storm 860 and Alpha Imagers This award will fund the development of an interdepartmental facility for documentation and quantification of biochemical and molecular biological data. This facility will provide equipment and software to digitize data derived from most gel electrophoresis experiments and allows analysis of the resulting data. The procedures and techniques that can be analyzed will include: DNA and RNA sequencing and structure analysis, PCR-based assays including quantitative PCR and RFLP analysis, quantitative northern and Western blotting, Southern hybridizations, non-radioactive detection for research and teaching laboratories, colony counting and kinetic analyses of enzymatic reactions. The goal is to maximize use by making the facility as flexible and as accessible as possible.Equipment to be purchased includes two imagers capable of quantifying data imaged using autoradiography, fluorescent stains or labels, visible stains or labels, or simply using white lighting. These two components are the Storm 860 Imager (Molecular Dynamics) and the AlphaImager (AlphaInnotech). Sufficient computational equipment will be purchased to perform image and data analysis. The facility will be installed on the first floor of the centrally located Biochemistry building, which will provide easy access to researchers in several departments. The facility will be used for undergraduate and graduate level training in quantifying and analyzing data collected from biological assays.