SBIR Phase II: Expression Pattern Screening for Agriculture Genomics

这个小企业创新研究（SBIR）第二阶段项目将使用一种新型的高通量平台，包括许多小基因阵列，包含在微量滴定板的威尔斯孔内。 该平台被称为多阵列板筛选（MAPS），其允许在96孔板的每个孔内使用源自96个单独样品的RNA同时测试特定组的感兴趣基因和适当对照的表达。MAPS为高通量筛选提供了终点检测，研究人员可以在其中评估不同的化合物如何应用于体内细胞、组织或生物体，这项技术将解决农业产业尚未满足的需求，即以不需要分发转基因生物的方式有效利用新的基因组学信息。以转基因植物的形式。使植物在96孔板的每个孔中生长以促进高通量筛选。该平台允许对包括新鉴定的基因在内的基因靶标进行简便有效的测试，并提供有关选择性和特异性的重要信息。 该项目的商业潜力在于农业市场。

This Small Business Innovation Research (SBIR) Phase II project will use a novel high throughput platform comprising of many, small gene arrays, contained within the wells of microtiter plates. This platform, termed Multi-Array Plate Screening (MAPS), allows simultaneous testing of the expression of a specific group of genes of interest and appropriate controls using RNA derived from 96 separate samples, within each well of a 96-well plate. MAPS provides the endpoint assay for a high throughput screen, in which investigators can evaluate how different chemical compounds, applied to cells, tissues, or organisms in vivo, affect the expression pattern for the genes of interest.This technology will address an unmet need of the agricultural industry to make efficient use of novel genomics information in a manner that does not require distribution of genetically modified organisms (GMOs) in the form of transgenic plants. The plants are grown in each well of a 96 well plate to facilitate high-throughput screening. The platform allows facile and efficient testing of gene targets including newly identified genes, and also provides important information about selectivity and specificity. The commercial potential from this project is in the agricultural market.