Purchase of a MALDI-TOF Mass Spectrometer

在化学研究仪器和设施（CRIF）计划的支持下，亚利桑那州立大学化学和生物化学系将获得一台基质辅助激光解吸电离-飞行时间（MALDI-TOF）质谱仪。 这一仪器将促进若干领域的研究：a）有机化合物的合成，包括以卟啉为基础的大型复合物; B）糖基化类胡萝卜素粘叶黄素生物合成所涉及的中间体和产物的特性; c）经历翻译后修饰的蛋白质的特性; d）合成DNA结合多肽的特性;以及e）降解酚类化合物的胞外酶的蛋白质组学研究。 基质辅助激光解吸电离（MALDI）飞行时间（TOF）质谱是获得高达和超过300，000道尔顿的分子的精确分子量的首选技术，具有极高的灵敏度。 因此，MALDI-TOF质谱仪的使用已成为大多数DNA/蛋白质核心设施中的标准技术。 获得非常精确的生物分子分子量的能力是所有生物学和生物医学研究的基础，也是现代研究和培训环境中必不可少的。

With support from the Chemistry Research Instrumentation and Facilities (CRIF) Program, the Department of Chemistry and Biochemistry at Arizona State University will acquire a Matrix-assisted Laser Desorption Ionization - Time of Flight (MALDI-TOF) Mass Spectrometer. This instrument will facilitate research in a number of areas: a) the synthesis of organic compounds, including large porphyrin-based complexes; b) characterization of intermediates and products involved in the biosynthesis of the glycosylated carotenoid myxoxanthophyll; c) characterization of proteins that have undergone posttranslational modifications; d) characterization of synthetic DNA binding polypeptides; and e) proteomics study on extracellular enzymes that degrade phenolic comopounds. Matrix-assisted laser desorption ionization (MALDI) time-of-flight (TOF) mass spectrometry is the technique of choice for obtaining accurate molecular weights on molecules up to and over 300,000 daltons, with extremely high sensitivity. Use of a MALDI-TOF mass spectrometer has therefore become a standard technique in most DNA/protein core facilities. The ability to obtain very accurate molecular weights of biomolecules is fundamental to all biological and biomedical research and essential in a modern research and training environment.