NIRT: Bioavailability of Aromatic Hydrocarbons and Dynamics of Their Interactions with Humic and Fulvic Acids: Linking Molecular- and Microbial-Scale Interactions

0210839 Nanny该提案是响应纳米科学与工程倡议NSF 01-157，类别NIRT而收到的。 该项目将确定纳米尺度的关键物理，化学和生物条件，这些条件决定了与土壤，沉积物和垃圾填埋场分离的NOM相关的芳烃的生物利用度。 在NOM表征和定量后，将评估局部分子尺度相互作用、%烃残留和相互作用活化能对生物利用度的影响。 萘双加氧酶活性将被表征为NOM类型和浓度以及NOM-芳香老化的函数。 将使用生物发光报告与微生物生物利用度进行耦合。 这项研究的结果将是开发基于NMR的表征，这将与酶测定和微生物生物测定相关。

0210839 Nanny This proposal was received in response to the Nanoscale Science and Engineering Initiative NSF 01-157, category NIRT. The project will identify key physical, chemical and biological conditions at the nano-scale that govern the bioavailability of aromatic hydrocarbons associated with NOM isolated from soils, sediments, and landfills. After NOM characterization and quantitation, the impact of the localized molecular scale interactions, % hydrocarbon residue, and activation energy of interactions will be assessed with respect to bioavailability. Naphthalene dioxygenase activity will be characterized as a function of NOM type and concentration and NOM-aromatic aging. Coupling to microbial bioavailability will be made using bioluminescence reporting. An outcome from the research will be the development of NMR based characterizations that will correlate with an enzymatic assay and a microbial bioassay.