SBIR Phase I: Innovative Protein Microarrays

这个小企业创新研究（SBIR）第一阶段项目提出应用多光子检测技术（MPD）来量化与抗体微阵列结合的蛋白质。第一批阵列将靶向细胞因子，这是调节免疫系统的重要分泌蛋白。本项目的目标是建立一种基于免疫分析的蛋白质芯片，用于同时测量所有细胞因子，低至几个zeptomole/样品的水平，使用肽核酸接头实现改进的P-芯片设计，并研究细胞培养物和人体样品中细胞因子的水平。通过分析蛋白质的表达模式及其翻译后修饰，可以建立功能和/或疾病状态与特定蛋白质表达模式的相关性，该项目的商业应用是在医学诊断领域。

This Small Business Innovation Research (SBIR) Phase I project proposes to apply Multi Photon Detection technique (MPD) to quantify proteins bound to a microarray of antibodies. The first arrays will target cytokines, the important secreted proteins that regulate the immune system. The goals of this project are to create an immunoassay based protein-chip for the concurrent measurment of all the cytokines down to the level of a few zeptomole/ sample, to implement improved P-chip designs using peptide nucleic acid linkers, and to study the levels of cytokines in cell cultures and human samples. By analyzing patterns of protein expression and their post-translational modifications, correlations of functions and/or of disease states with specific protein expression patterns are expected to be established.The commercial applications of this project are in the area of medical diagnostics.