QSB: Development of an Integrated Biological and Computational Approach to Decipher Transcriptional Regulatory Networks in Saccharromyces Cerevisiae

该项目的长期目标是开发一种综合的生物学和计算方法来破译基因调控网络。 该项目结合发现为基础的方法，以确定和表征新的监管网络与假设驱动的方法，其中转录控制的两个重要的半乳糖代谢调控因子将被检查。 该项目的具体目标是：（1）在时间上分析全球遗传和蛋白质组对半乳糖的反应;（2）在全球范围内识别对半乳糖利用重要的转录网络的元素;（3）开发一个先进的计算框架，用于整合，建模和预测转录网络;（4）实验测试这些研究预测的网络模型。

The long range goal of this project is to develop an integrated biological and computational approach to decipher gene regulatory networks. This project combines discovery-based approaches to identify and characterize new regulatory networks with hypothesis-driven approaches in which transcriptional control by two regulatory factors important in galactose metabolism will be examined. The specific aims of this project are: (1) to temporally profile the global genetic and proteomic response to galactose; (2) to globally identify the elements of transcriptional networks important for galactose utilization; (3) to develop an advanced computational framework for integration, modeling, and prediction of transcriptional networks; and (4) to experimentally test the networks models predicted from these studies.