NER: A Novel Nanobiosensor Architecture

摘要我们建议开发一种新的纳米生物传感器结构，该结构基于金纳米线在商用聚合物膜上的化学沉积，然后是化学机械平面化（CMP），这是一种广泛应用于半导体工业的加工步骤，用于生产极其光滑的表面。光滑的表面是必要的，以便使用特定的化学物质将酶和其他蛋白质附着在生物传感器表面。这个表面可以用原子力显微镜（AFM）的尖端来绘制图案，允许不同的蛋白质附着在不同的区域。最终目标是将纳米线阵列无缝嵌入聚碳酸酯膜中，该膜可以进行图案设计，以允许平行检测不同的分子。这种新的纳米生物传感器结构将通过将葡萄糖氧化酶固定在金纳米线上来验证。该葡萄糖生物传感器的检测限将通过测量不同葡萄糖浓度溶液中的电化学电流来确定。此外，这种生物传感器将使用更先进的电化学方法进行测试，该方法也可以测量表面电容。与其他电化学生物传感器相比，这种纳米生物传感器结构具有许多优点。这些包括单分子检测和小型化的能力，生物相容性以及与许多不同检测方法的兼容性。这项研究的影响可能相当广泛，有可能开发新一代金/聚碳酸酯生物传感器，包括监测生物体的传感器。这可能会在临床诊断、医疗植入物、环境监测、国土安全和食品工业等领域取得重大进展。这项研究的结果将广泛传播，包括互联网，使用PI的多媒体创作专业知识，这是通过国家科学基金会资助的课程开发项目开发的。

Proposal No: 304143Title: NER: A Novel Nanobiosensor ArchitectureAbstract We propose to develop a new nanobiosensor architecture based on chemical deposition of gold nanowires into a commercially available polymer membrane, followed by chemical mechanical planarization (CMP), a processing step widely employed in the semiconductor industry for producing extremely smooth surfaces. A smooth surface is necessary to allow the use of specific chemistries to attach enzymes and other proteins to the biosensor surface. This surface can be patterned using the tip of an atomic force microscope (AFM), allowing different proteins to be attached in different areas. The ultimate goal is a nanowire array seamlessly embedded into a polycarbonate membrane that can be patterned to allow parallel detection of different molecules.This new nanobiosensor architecture will be validated by immobilizing glucose oxidase onto the gold nanowires. The detection limit of this glucose biosensor will then be determined by measuring the electrochemical current in solutions of varying glucose concentration. In addition, this biosensor will be tested using more advanced electrochemical methods that can also measure the surface capacitance. This nanobiosensor architecture has a number of advantages relative to other electrochemical biosensors. These include the capability for single molecule detection and for miniaturization, biocompatibility, and compatibility with many different detection methods. The impact of this research is potentially quite broad, with the possible development of a new generation of gold/polycarbonate biosensors, including sensors for monitoring living organisms. This could lead to significant advances in the fields of clinical diagnostics, medical implants, environmental monitoring, homeland security, and the food industry. The results of this research will be broadly disseminated, including the Internet, using the PI's multimedia authoring expertise, which was developed through NSF-sponsored curriculum development projects.