RUI MRI Genomic and Genetic Analysis Research in Molecular Biology at Pomona College

在劳拉·胡普斯博士的指导下，波莫纳学院获得了一笔资助，以促进基因组和基因分析研究。这笔拨款将为购买微阵列扫描仪、相关计算机和软件、磷光成像仪和实时PCR序列检测系统提供资金。所有这些仪器将用于波莫纳学院5个不同实验室的师生合作研究。微阵列扫描仪将用于检测酵母衰老过程中的基因表达变化，通过识别相似DNA的短序列来影响酵母重组能力的突变体，以及生长在海底热喷口中的古细菌。此外，微阵列扫描仪将用于寻找克隆到大肠杆菌中的归巢内切酶（及其突变版本）菌株的基因表达变化的项目。它还将用于检测果蝇发育突变体的基因表达，这些突变体会破坏细胞内膜封闭囊泡的运输。实时PCR将用于定量微阵列分析的结果，并检查质粒的双链断裂（DSB）修复。该磷光成像仪将用于师生研究，以检查dsb的产生和修复，以及芯片分析。磷成像仪还将用于定量各种DNA复制结构的数量，这些结构可以使用二维凝胶电泳检测到，使我们能够分析老化细胞与突变细胞的复制叉停滞。这笔拨款将以多种方式影响科学基础设施。波莫纳学院的许多学生将获得前沿遗传学和基因组学研究方面的经验，从而增强他们在这些领域从事职业的兴趣。微阵列扫描仪和磷光成像仪将促进波莫纳学院真核生物遗传调控和其他研究型课程的主要实验，学生将学习这些技术的背景，并将他们的知识应用于实验室的实际问题。最后，微阵列扫描仪将能够扫描主动教学基因组联盟（GCAT）西海岸成员，其本科生研究项目和研究型课程每年影响100多名学生。

A grant has been awarded to Pomona College under the direction of Dr. Laura Hoopes to facilitate genomic and genetic analysis research. The grant will provide funds for the acquisition of a microarray scanner, associated computers and software, a phosphoimager, and a sequence detection system for real-time PCR. All of these instruments will be used in faculty-student collaborative research in 5 different laboratories at Pomona College. The microarray scanner will be used to examine gene expression changes in yeast aging, in mutants affecting the ability of yeast to recombine by recognizing short sequences of similar DNA, and in archaebacteria that grow in hot vents on the ocean floor. In addition, the microarray scanner will be used in projects to look for changes in gene expression in strains with a homing endonuclease (and mutant versions of it) cloned into the bacterium Escherichia coli. It will also be used to examine gene expression in developmental mutants of Drosophila that disrupt transport of membrane-enclosed vesicles within cells. Real-time PCR will be used to quantitate the results of microarray analyses, and to examine plasmids for double strand break (DSB) repair. The phosphoimager will be used in faculty-student research to examine the creation and repair of DSBs, and in ChIP assays. The phosphoimager will also be used to quantitate the amounts of various DNA replication structures that can be detected using 2-dimensional gel electrophoresis, allowing us to analyze replication fork stalling in aged vs. mutant cells.There are a number of ways this grant will impact the scientific infrastructure. Many students at Pomona College will gain experience in cutting-edge genetics and genomics research, thus reinforcing their interest in pursuing careers in these fields. The microarray scanner and phosphoimager will facilitate a major experiment in the Genetic Regulation in Eukaryotes and other research-style classes at Pomona College, where students will learn the context of these techniques, as well as apply their knowledge to a real problem in the laboratory. Finally, the microarray scanner will enable scanning for the Genome Consortium for Active Teaching (GCAT) West Coast members, whose undergraduate student research projects and research-oriented courses impact more than 100 students annually.