Collaborative Research: Mechanism of Enhanced Biological Phosphorus Removal

拟议工作的一个广泛目标是确定微生物群落结构和功能如何与成功的EBPR过程性能相关联。为了实现这一目标，必须研究有效的EBPR操作所必需的关键生物。特别是，有必要研究这些生物如何调节和控制参与EBPR代谢的基因和基因产物。这项工作的具体目标是：直接从富含pao的污泥的混合群落中克隆参与PHA和糖原循环的基因；阐明PAOs中碳和磷循环发生的机制；建立一种检测污泥中息肉代谢的免疫化学方法；整合观察结果并推荐全尺寸epbr系统的潜在控制策略。

0332136 McMahon A broad objective of the proposed work is to determine how microbialcommunity structure and function is linked to successful EBPR process performance. To achieve this objective, the key organisms whose presence is necessary for efficient EBPR operation must be studied. In particular, it is necessary to investigate how these organisms regulate and control the genes and gene products involved in EBPR metabolism. Specific goals of this work are to:oClone the genes involved in PHA and glycogen cycling directly from mixedcommunities in PAO-enriched sludges;oElucidate the mechanisms by which carbon and P cycling occurs in PAOs;oDevelop an immunochemical method to detect polyP metabolism in sludge;oIntegrate observations and recommend potential control strategies for full-scaleEPBR systems.