Cold Spring Harbor Laboratory ADVANCED BACTERIAL GENETICS COURSE

冷泉港实验室建议继续其非常成功的暑期高级细菌遗传学课程。这门课程起源于1945年由Max Delbruck博士发起的噬菌体课程。这个为期三周的课程为参与者准备直接进入研究，利用分子遗传学和微生物生物信息学的先进技术和概念。为经典遗传分析提供坚实基础的实验支持转座子诱变、基因融合技术、染色体作图、缺失作图和互补分析、第二位点抑制分析和全基因组分析的当代应用培训。实验还向学生介绍了基因克隆的新分子技术，位点定向诱变的新进展，DNA微阵列分析和聚合酶链反应应用于细菌和噬菌体遗传学的各种问题，通过转座子插入端测序和基因组序列搜索来“定位”基因，以及使用转录、翻译和细胞定位报告连同指示介质和反褶积显微镜识别突变体。这门课程在提供遗传学和分子生物学的指导方面是独一无二的，它为参与者提供了在他们的家庭实验室进行类似实验所需的必要资金。

The Cold Spring Harbor Laboratory proposes to continue its highly successful summer course in Advanced Bacterial Genetics. This course has its roots in the original Bacteriophage Course initiated by Dr. Max Delbruck in 1945. This three week course prepares the participant to enter directly into research that makes use of advanced techniques and concepts in molecular genetics and microbial bioinformatics. Experiments providing a solid grounding in classical genetic analysis support training in contemporary uses of transposon mutagenesis, gene fusion technology, chromosomal mapping, deletion mapping and complementation analysis, second-site suppressor analysis and whole genome analysis. Experiments also introduce the student to new molecular techniques of gene cloning, new advances in site-directed mutagenesis, DNA microarray analysis and polymerase chain reaction as applied to diverse problems in bacterial and bacteriophage genetics, "mapping" genes by sequencing from transposon insertion ends and genome sequence searching, and the use of transcriptional, translational and cellular localization reporters along with indicator media and deconvolution microscopy to identify mutants. This course, which is unique in providing instruction in both genetics and molecular biology, gives participants the wherewithal required to perform analogous experiments in their home laboratories.