NER: Two-Dimensional Sol Gel Protein Imprinting

Britt0404262本研究旨在开发一种二维印迹和分子钳方法来创建用于蛋白质识别的纳米级溶胶凝胶膜。平面内印迹位置形成在可聚合的烷基硅烷与功能化的目标硅烷和聚乙二醇脂类共铺展的浮动单分子层中。单层组分的横向扩散性有助于通过互补相互作用(氢键、静电)将两亲性官能团与吸附蛋白质上的残基匹配，这种相互作用在单层聚合时得到保留。朗缪尔单分子膜技术可以精确控制官能团密度、侧向迁移率、聚合速率、凝胶膜形态、聚乙二醇单分子构象和蛋白质插入。印迹单分子层在烷基硅烷水解和缩合过程中的收缩，伴随着势垒压缩，为印迹增加了一个“分子虎”方面，最初在“蘑菇”相中探索大片区域的聚乙二醇链被迫进入更有序和构象受限的“刷子”相。这种诱导聚乙二醇两亲性围绕被吸附到目标硅烷头基的蛋白质周围的组装建立了结合口袋，而弱吸附的蛋白质(较差的印迹)被挤出，改善了结合部位的亲和力和均一性。面内印迹周围的聚乙二醇刷状层也减少了非印迹介导的蛋白质重新结合。拟议的合作研究将有助于在犹他州立大学生物工程系和犹他大学生物工程和冶金工程系之间建立基于纳米科学的合作伙伴关系，从而加强基础设施并促进两所大学的研究生和本科生的合作

Britt0404262This research aims to develop a two-dimensional imprint and molecular vise method for creating nanoscale sol-gel membranes for protein recognition. In-plane imprint sites are formed in floating monolayers of polymerizable alkyl-silanes co-spread with functionalized "target" silanes and poly(ethylene glycol) (PEG) bearing lipids. The lateral diffusivity of monolayer components facilitates a matching of amphiphile functional groups with residues on the adsorbing proteins through complementary interactions (hydrogen bonding, electrostatic), preserved upon monolayer polymerization. The Langmuir monolayer technique allows functional group density, lateral mobility, polymerization rate, gelled film morphology, PEG conformation, and protein insertion to be precisely controlled. Contraction of the imprinted monolayer upon alkyl-silane hydrolysis and condensation accompanied by barrier compression adds a "molecular vise" aspect to imprinting, where PEG chains that initially explore a large area in the "mushroom" phase are forced into the more ordered and conformationally restricted "brush" phase. This induced assembly of PEG bearing amphiphiles around proteins adsorbed to target silane head-groups builds binding pockets, while weakly adsorbed proteins (poor imprints) are squeezed out, improving binding site affinity and homogeneity. The PEG brush layer surrounding the in-plane imprints also reduces non-imprint mediated protein re-binding. The proposed collaborative research will help establish a nanoscience based partnership between the Biological Engineering department at Utah State University and the Bioengineering and Metallurgical Engineering departments at the University of Utah, leading to infrastructure enhancement and cooperative graduate and undergraduate student involvement from both universities