Methodology for Rapid NMR Data Acquisition in Structural Genomics

该项目的重点是通过扩展适用于13 C/15 N稳定同位素标记的蛋白质和核酸的GFT NMR实验的全部内容，并开发结合单扫描采集方案的GFT NMR实验，实现多维NMR数据的快速收集的新方法。为了进一步降低GFT NMR的采样要求，将通过使用最大熵重构或三向分解来优化GFT NMR数据的处理。新的NMR实验的用户友好就业将通过实施软件来确保GFT NMR谱的有效分析。建立一套核磁共振参数将有助于有效传播研究成果。这些活动产生的更广泛的影响是提高了基于核磁共振的结构生物学和基因组学的效率，增加了对昂贵的高端核磁共振硬件和基因组测序项目的投资回报。该研究计划将为学生提供机会。

This project focuses on the implementation of novel methodology for rapid collection of multidimensional NMR data by extending the repertoire of GFT NMR experiments suitable for 13C/15N stable isotope labeled proteins and nucleic acids and developing GFT NMR experiments that incorporate the single scan acquisition scheme. To further reduce the sampling demand of GFT NMR, processing of GFT NMR data will be optimized by using either Maximum Entropy Reconstruction or Three-way Decomposition. User-friendly employment of the new NMR experiments will be ensured by implementing software for efficient analysis of GFT NMR spectra. The creation of a package of NMR parameter sets will enable efficient dissemination of research results. The broader impacts resulting from these activities are increased efficiency with which NMR-based structural biology and genomics can be pursued and an increase in the return of investment into costly high-end NMR hardware and genome sequencing projects. The research program will offer opportunities to students.