Genetic analysis of protein kinase C signaling in ion flow-directed cell movement

材料的表面特性可以极大地影响接触细胞的行为，这是设计治疗性植入物时的一个重要因素。除了物理化学性质外，最近的研究表明，表面电学性质直接影响细胞-材料界面的生物反应。除了众所周知的生理功能外，细胞膜上的离子转运蛋白还能产生小电场。已知内源性和外源性电场显著影响各种生物过程，如伤口愈合、肿瘤转移、免疫反应和组织形成/再生。所有这些事件都有一个共同的基本组成部分：定向细胞运动。电场可以通过离子通量触发各种细胞信号级联反应。在某些情况下，如伤口愈合，电场似乎是引导细胞迁移的最强大的定向线索，超过了包括趋化性在内的其他线索。细胞如何感知EF的方向仍不清楚。为了帮助基础研究和基于治疗的生物工程方法的发展，因此了解内源性和外源性电场影响生物系统的机制是必不可少的。

Surface properties of a material can greatly affect the behaviour of contacting cells, an important factor when designing therapeutic implants. In addition to physicochemical properties, recent studies suggest that surface electrical properties directly affect biological responses at the cell-material interface. Beyond their wellknown physiological functions, ion transport proteins in the cell membrane generate small electric fields. Endogenous as well as exogenous electric fields are known to significantly influence various biological processes, such as wound healing, tumor metastasis, immune responses and tissue formation/regeneration. All these events have a common essential component: directional cell motility. Electric fields can trigger a variety of cellular signalling cascades via ion fluxes. In certain conditions such as wound healing, electric fields appear to be the most powerful directional cue guiding cell migration, overriding other cues including chemotaxis. How cells sense the direction of an EF remains unclear. In order to aid the development of both basic research and therapy-based bioengineering approaches, it is thus essential to understand the mechanisms through which endogenous and exogenous electric fields impact biological systems.