LTREB: Relatedness Asymmetries, Antagonistic Natural Selection and Nonmendelian Inheritance in a Natural Population of Lizards

现有的基因组印记证据仅限于从自然选择的影响中分离出来的模式生物。这项建议中描述的实验代表了对野生脊椎动物基因组印记的开创性研究，这将为自然选择如何塑造自然界中的遗传变异和遗传模式提供有价值的和普遍的见解。继续对侧斑蜥蜴的母性和父性进行评估，将产生野外最广泛的家系之一。对这一自然系统生物学的日益深入的理解导致了基因组印记进化的新假说的发展，这一假说将得到明确的检验。只有通过将这一广泛的谱系与优雅的育种设计和对母体效应的实验操作相结合，这些令人兴奋的研究现在才成为可能。更广泛的影响：这项研究将培养一名博士后研究员、两名研究生和20名本科生，其中包括几名女性和少数族裔。共同派·麦克亚当是一名初级教员，这个项目将为他的研究计划提供一个有价值的开始。更广泛的公众宣传将通过一个互动网站面向K-12年龄段，该网站将教授科学方法、自然历史和进化论。这项研究还将制作光盘材料，供K-12教育工作者在订阅情况良好的科学教育网站(http://wise.Berkeley.edu).)上使用这些外展工作将为社会提供对自然历史和进化过程的更深层次的了解

Existing evidence of genomic imprinting has been limited to model organisms isolated from the effects of natural selection. The experiments described in this proposal represent a pioneering investigation of genomic imprinting in a wild vertebrate that will provide valuable and general insights into the ways in which natural selection shapes patterns of genetic variation and inheritance in nature. The continued assessment of both maternity and paternity for side-blotched lizards will generate one of the most extensive field pedigrees in the wild. Increasing understanding of the biology of this natural system has led to the development of a novel hypothesis for the evolution of genomic imprinting, which will be explicitly tested. It is only through the integration of this extensive pedigree with elegant breeding designs and experimental manipulations of maternal effects that these exciting investigations are now possible. Broader Impacts: The research will train one postdoctoral fellow, two graduate students and twenty undergraduates, including several women and minorities. Co-PI McAdam is a junior faculty member and this project will provide a valuable start to his research program. Broader public outreach will be targeted at the K-12 age group through an interactive website that will teach about the scientific method, natural history and evolution. The research will also produce material for a CD Rom that will be available for K-12 educators on a well-subscribed science education site (http://wise.Berkeley.edu). These outreach endeavors will provide society with a deeper understanding of natural history and the evolutionary process