SGER: Electrophile Responsive Proteome and Their Role in Plant Signaling Pathways

各种环境和发育刺激产生反应性电泳体(Res)，通过共价加合物的形成修饰蛋白质，并在动物系统中触发适应性细胞反应。尽管这种被称为电泳体介导的硫醇结合(EMTB)的翻译后修饰已知在动物中调节信号转导途径，但在植物系统中尚未见报道。最近发现，EMTB修饰是植物氧脂途径中的一个关键酶，在植物胁迫反应中起着重要的信号作用。这个项目的目标是研究EMTB修饰在控制植物信号通路中的一般作用。智力上的优点：跨学科的方法将被用来在全基因组范围内识别由胁迫诱导的12-氧代植物二烯酸(12-OPDA)共价修饰的细胞靶蛋白，12-OPDA是植物中的一种脂类。12-OPDA结合蛋白靶标的一个子集将被充分表征并研究它们在植物逆境信号通路中的作用。最后，将确定12-OPDA与其他应激诱导的硫代反应性亲电体在识别的蛋白质的共价修饰中的特异性。更广泛的影响：这项研究的结果将为控制植物中复杂而关键的信号网络与动物信号系统显著相关的一种新的、以前未被认识的翻译后调控机制提供重要的基础性见解。此外，由ETMB调控的途径组件的发现可能为在重要的农艺作物中产生生物技术应用提供工具。该项目还将为本科生和博士后提供最先进的跨学科研究方面的培训，并将包括努力招收未被充分代表的少数族裔本科生，在论文、研讨会和会议报告中广泛传播研究成果，以及开发关于翻译后修饰在信号通路中的作用的短期课程。

Various environmental and developmental stimuli generate reactive electrophile species (RES) that modify proteins through covalent adduct formation and trigger adaptive cellular responses in animal systems. Although this post-translational modification termed electrophile mediated thiol-binding (EMTB) is known to regulate signal transduction pathways in animals, it has not been previously reported in plant systems. Recently it was discovered that EMTB modification of a key enzyme in the oxylipin pathway of plants functions as an important signal in plant stress responses. The goal of this project is to examine the general role of EMTB modification in controlling plant signaling pathways.Intellectual Merit: Interdisciplinary approaches will be used to identify, on a genome-wide scale, the cellular target proteins that are covalently modified by stress-induced 12-oxophytodienoic acid (12-OPDA), a lipid RES in plants. A subset of the 12-OPDA binding protein targets will be fully characterized and examined for their role in plant stress signaling pathways. Finally, the specificity of 12-OPDA versus the other stress-induced thiolreactive electrophiles in covalent modification of the proteins identified will be determined. Broader impacts: The results of this study will provide important foundational insights into a novel, previously unrecognized posttranslational regulatory mechanism controlling a complex and crucial signaling network in plants with significant relevance to animal signaling systems. In addition the discovery of pathway components regulated by ETMB may provide for the generation of tools for biotechnological applications in agronomically important crops. This project will also provide for the training of undergraduate students and postdoctoral fellows in state-of-the-art interdisciplinary research and will include efforts to recruit underrepresented minority undergraduate students, the broad dissemination of research results in papers, seminars, and conference presentations, and the development of short courses on the role of post-translational modification in signaling pathways.