Defining transcriptional and post-translational regulatory networks in retrogradw stress signaling

定义逆行应激信号传导中的转录和翻译后调节网络

• 批准号: 1352478
• 负责人: Katayoon Dehesh
• 金额: $ 67万
• 依托单位: University of California-Davis
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-02-15 至 2016-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1352478&HistoricalAwards=false
• 关键词: Defining; transcriptional; post; translational; regulatory

Plants are persistently challenged with numerous biotic and abiotic stresses. To cope, they have evolved an intricate network of mechanisms to link these stress response pathways. Identification of master regulators that prime these response pathways is fundamental to providing opportunities for developing broad-spectrum stress-tolerant plants. Recently, it was discovered that a novel plastid-produced stress-specific signaling metabolite, methylerythritol cyclodiphosphate (MEcPP), serves as a sensory hub that synchronizes multiple regulatory steps that are key to plant responses to environmental perturbations. This discovery has led to construction of a unique platform for identification of the general stress response regulators. The aims of this project are two-fold: 1) to identify the molecular and biochemical components engaged in the MEcPP signaling cascade that relay plastid-perceived stress signals to a central hub responsible for expression of key regulators of biotic and abiotic stress responses. 2) to identify the cellular interactomes that might connect the integrative network of sensory systems required for plants adaptive responses to multiple environmental stresses. As such, this project provides a unique opportunity to unambiguously identify and functionally characterize an ensemble of a general stress cluster of genes key to plant adaptive responses to biotic and abiotic stresses. This project provides a multidimensional educational platform for mentoring scholars and particularly underrepresented college and high school students in a vertically integrated state-of-the-art interdisciplinary translational research. The results of this project will be stored daily on the departmental server and widely disseminated through publications and public presentations, and all of the tools developed will be made available to the community. The global impact of this project is the development of new targets for generation of broad-spectrum stress-tolerant crops, thereby alleviating pressures caused by climate change and global food security.

植物一直受到大量生物和非生物胁迫的挑战。为了应对，他们进化出了一个错综复杂的机制网络，将这些应激反应途径联系起来。确定启动这些反应途径的主要调控因子是为开发广谱耐逆植物提供机会的基础。最近，人们发现了一种新型的叶绿体产生的逆境信号代谢产物甲基赤藓糖醇环二磷酸(MECPP)，它作为一种感觉中枢，同步了植物对环境干扰反应的关键调控步骤。这一发现导致了一个独特的平台的构建，以识别一般的应激反应调节因子。该项目的目标有两个：1)确定参与MECPP信号级联的分子和生化成分，这些分子和生化成分将感受到的叶绿体逆境信号传递到负责表达生物和非生物逆境反应关键调节因子的中央枢纽。2)确定可能连接植物对多种环境胁迫的适应性反应所需的感觉系统的整合网络的细胞相互作用。因此，这个项目提供了一个独特的机会，可以明确地识别和功能描述对植物对生物和非生物胁迫的适应性反应至关重要的一般胁迫基因簇的集合。这个项目提供了一个多维的教育平台，在垂直整合的最先进的跨学科翻译研究中指导学者，特别是代表不足的大学生和高中生。这一项目的成果将每天存储在部门服务器上，并通过出版物和公开介绍广泛传播，开发的所有工具都将提供给社区。该项目的全球影响是为生产广谱耐逆作物制定了新的目标，从而缓解了气候变化和全球粮食安全造成的压力。