The plastidial MEP-pathway signature in mitochondrial structure and function

线粒体结构和功能中的质体 MEP 通路特征

• 批准号: 2104365
• 负责人: Katayoon Dehesh
• 金额: $ 85万
• 依托单位: University of California-Riverside
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-08-01 至 2024-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2104365&HistoricalAwards=false
• 关键词: plastidial; MEP; pathway; signature; mitochondrial

Mitochondria are known as the ‘power-house’ in both animal and plant cells. In plant cells, mitochondria are the site of respiration where fuel derived from photosynthesis in the chloroplasts is converted to energy currency. Mitochondria can move within the cell, and upon encountering each other undergo fusion, resulting in the merger of two mitochondria into a single larger mitochondrion. Conversely, a single mitochondrion can divide into two distinct mitochondria via fission. The balanced frequencies of fusion and fission determine the overall morphology of the mitochondrial population, and are crucial for maintaining mitochondrial functions such as respiratory capacity and response to stress signals. This project will use genetic manipulations of a biochemical route (the “MEP pathway”) used to respond to stress, in order to uncover how alteration of a chloroplast’s metabolism affects the genes and proteins that determine the shape and activity of mitochondria. The outcome of this research will determine the potential signaling functions of chloroplasts through the MEP-pathway and determine if chloroplasts affect mitochondria directly, or if the signal must first travel through the cell nucleus in order to remodel mitochondrial shape and function. These results could be used to help plants become more resistant to parasites, and tolerant of growth in dry or other stressful areas. This project will also provide laboratory training for undergraduate and graduate students as well as postdoctoral fellows; and will expose high school students to the study of plant biochemistry and its importance to the growth of plants and role in food production.Although both the mevalonic acid pathway and MEP pathway synthesize plant isoprenoids, the MEP pathway is indispensable to plant growth, which suggests that it has other functions. Mutants in the MEP pathway give rise to cells with altered mitochondrial fission and fusion rates leading to altered morphologies. This project will investigate communication between chloroplast and mitochondria via the MEP-pathway through two Aims: A) Alter specific steps of the MEP pathway and examine mitochondrial morphology via biochemistry, genetics, and fluorescent and electron microscope tomography; B) Use physiological, genetic, and transcriptomics techniques to investigate which steps of mitochondrial morphogenesis are perturbed. This work will provide fundamental insights into the molecular and biochemical links required for functional coordination of two interdependent types of organelles in plants, providing greater understanding of interorganellar communication in all eukaryotic cells as well as understanding of biochemistry of plant resistance to harsh conditions. In addition, the PI will bring local students from five nearby underfunded high schools into the laboratory for yearlong training. The PI as well as students and postdoctoral fellows in the laboratory will also participate in organizing and judging local science fairs.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

线粒体被称为动物和植物细胞中的“动力室”。 在植物细胞中，线粒体是呼吸作用的场所，叶绿体中光合作用产生的燃料在此转化为能量货币。 线粒体可以在细胞内移动，并且在彼此相遇时发生融合，导致两个线粒体合并成一个更大的线粒体。 相反，单个线粒体可以通过裂变分裂成两个不同的线粒体。 融合和裂变的平衡频率决定了线粒体群体的整体形态，对于维持线粒体功能（例如呼吸能力和对应激信号的反应）至关重要。 该项目将利用用于应对压力的生化途径（“MEP 途径”）的基因操作，以揭示叶绿体代谢的改变如何影响决定线粒体形状和活性的基因和蛋白质。 这项研究的结果将确定叶绿体通过 MEP 途径的潜在信号传导功能，并确定叶绿体是否直接影响线粒体，或者信号是否必须首先穿过细胞核才能重塑线粒体的形状和功能。 这些结果可用于帮助植物增强对寄生虫的抵抗力，并耐受干燥或其他压力地区的生长。 该项目还将为本科生、研究生以及博士后提供实验室培训；将使高中生接触植物生物化学的研究及其对植物生长的重要性以及在粮食生产中的作用。虽然甲羟戊酸途径和MEP途径都合成植物类异戊二烯，但MEP途径对于植物生长是不可或缺的，这表明它还有其他功能。 MEP 途径中的突变体会产生线粒体裂变和融合率改变的细胞，从而导致形态改变。 该项目将通过两个目标研究叶绿体和线粒体之间通过 MEP 途径的通讯：A) 改变 MEP 途径的特定步骤，并通过生物化学、遗传学、荧光和电子显微镜断层扫描检查线粒体形态； B) 使用生理、遗传和转录组学技术来研究线粒体形态发生的哪些步骤受到干扰。 这项工作将为植物中两种相互依赖类型的细胞器的功能协调所需的分子和生化联系提供基本见解，从而更好地了解所有真核细胞中的细胞器间通讯以及植物抵抗恶劣条件的生物化学。 此外，PI还将把附近五所资金不足的高中的当地学生带到实验室进行为期一年的培训。 PI以及实验室的学生和博士后研究员也将参与当地科学博览会的组织和评审。该奖项反映了NSF的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。