Development and Application of On-Line cITP-NMR for Analysis of Mass-Limited Samples

加州大学河滨分校的Cynthia K. Larive教授在化学系分析和表面化学项目的支持下，致力于毛细管等速电泳-核磁共振（ctp - nmr）方法的开发，并将这种浓度、分离和双重检测分析工具应用于质量有限样品和低聚糖肝素的分析和结构测定。分析物带将通过电导率检测，并将带聚焦在NMR微线圈探针中，以允许信号平均和二维NMR实验。低水平杂质的检测和结构确定是药物开发和发现的关键组成部分，使用ctp -NMR将显著减少分离寡糖和其他化学物质和生物化学物质所需的核磁共振分析的质量。这项研究将推动在线ctp - nmr仪器的发展，并将对分析化学、生物化学和制药工业产生影响。此外，该项目将教育学生，包括本科生和代表性不足的群体成员，了解最先进的分析仪器和方法。

Professor Cynthia K. Larive of the University of California, Riverside is supported by the Analytical and Surface Chemistry Program in the Division of Chemistry to work on the development of capillary isotachophoresis- Nuclear magnetic resonance (cITP-NMR) methodology, and to apply this concentration, separation, and dual detection analytical tool for the analysis and structural determination of mass limited samples and heparin oligosaccarides. Analyte bands will be detected by conductivity, and the bands will be focused in the NMR microcoil probe to permit signal averaging and 2D NMR experiments. Detection and structure determination of low-level impurities is a critical component of drug development and discovery, and the use of cITP-NMR will appreciably reduce the mass of isolated oligosaccharides and other chemicals and biochemicals required for NMR analysis. This research will advance the instrumentation for on-line cITP-NMR and will impact analytical chemistry, biochemistry and the pharmaceutical industry. In addition, this project will educate students, including undergraduates and members of underrepresented groups in state of the art analytical instrumentation and methodology.