2010 Arabidopsis/AFGN Collaborative Project: An Exemplary Calcium Signaling Network in Plant Stress Responses

2010年拟南芥/AFGN合作项目：“植物非生物胁迫反应中的一个典型的钙信号网络”US-PI：盛滦，加州大学伯克利分校，美国；德国-PI：Jorg Kudla，德国明斯特大学；Cacineurin B-like钙传感器(CBL)及其相互作用的蛋白激酶(CIPK)，统称为CBL-CIPK网络。公众可以从哪里获得该项目的信息：确定功能的http://www.uni-muenster.de/Biologie.Botanik/agkudla/kudla.htmlDefinition：研究人员最近的研究发现了一系列新的钙传感器(CBL)，这些传感器针对一系列蛋白激酶(CIPK)，为植物中的钙信号建立了一个新的范例。由NSF和DFG支持的Luan-Kudla合作研究已经建立了一个广泛的CBL-CIPK网络，该网络在许多信号通路中发挥作用，包括对非生物胁迫的反应、营养感知和ABA反应。本研究旨在系统分析CBL-CIPK相互作用网络的机制和功能，以及它在非生物胁迫反应中对各种钙信号整合和解码的贡献。功能分析结果将通过上述网页和出版物分享。意义：植物中几乎所有的信号转导过程都涉及到作为重要第二信使的钙离子。了解Ca~(2+)如何介导由各种环境信号触发的细胞反应是植物生物学家未来几年最重要的目标之一。除了揭示植物对环境胁迫因子耐受性的分子基础外，这项研究还将深入了解与所有真核生物相关的钙信号机制，为植物生物学中蛋白质-蛋白质相互作用、突变分析和细胞生物学研究提供新的工具。更广泛的影响：除了对基础科学机构做出贡献外，这项研究还将影响社会和教育。这项研究中的发现可以通过六安实验室正在进行的多国合作应用于作物改良。这项研究旨在提高作物的养分吸收和抗逆性，特别是作为谷物模式的水稻。这项研究将通过PI在伯克利教授的主要课程和PI实验室的独立研究计划，对本科生和研究生教育产生额外的影响。NSF项目还将加强正在进行的以当地高中为重点的努力，以鼓励少数民族学生获得高等教育并进入生物专业。此外，美国和德国的合作将为培训学生和博士后进行国际合作提供一个重要的平台，这是当今地球村氛围下教育的一个重要组成部分。

2010 Arabidopsis/AFGN Collaborative Project: "An exemplary calcium signaling network in plant abiotic stress responses"US-PI: Sheng Luan, University of California at Berkeley, USAGerman-PI: Jorg Kudla, Universitat Munster, GermanyThe calcineurin B-like calcium sensors (CBLs) and their interacting protein kinases (CIPKs), collectively named CBL-CIPK network. URL where the public can get information on the project: http://www.uni-muenster.de/Biologie.Botanik/agkudla/kudla.htmlDefinition of Determining the Function: Recent studies by the investigators uncovered a new family of Ca2+ sensors (CBLs) that target a family of protein kinases (CIPKs), establishing a novel paradigm for Ca2+ signaling in plants. The Luan-Kudla collaborative research supported by NSF and DFG has established an extensive CBL-CIPK network that functions in a number of signaling pathways including responses to abiotic stress, nutrient sensing, and ABA responses. This research seeks to perform a systems analysis of the mechanisms and functions of CBL-CIPK interaction network and its contribution to the integration and decoding of various calcium signals during abiotic stress responses.The functional analysis results will be shared through the webpage above and publications as the results come along. Significance: Almost all signal transduction processes in plants involve Ca2+ that serves as a vital second messenger. Understanding how Ca2+ mediates the cellular responses triggered by myriad environmental signals is one of the most important goals for plant biologists in years to come. Besides shedding light on the molecular basis for plant tolerance to environmental stress factors, this research will produce insights into calcium signaling mechanisms with relevance for all eukaryotes, provide novel tools for protein-protein interaction, mutant analyses, and cell biology research in plant biology. Broader Impact: In addition to contributing to the body of fundamental science, this research will impact society as well as education. The findings made in this research can be applied to crop improvement via ongoing multi-national collaborations in the Luan laboratory. This research is designed to improve the nutrient uptake and stress tolerance of crop plants especially rice that serves as a model for cereals. The research will have an additional effect on undergraduate and graduate education through major courses the PI teaches at Berkeley and through independent research programs in PI's laboratory. The NSF project will also enhance ongoing efforts focused on local high schools to encourage minority students to obtain a higher education and enter biology. Furthermore, the US-Germany collaboration will provide a critical platform for training students and postdocs in international collaboration, a crucial component of education in today's global village atmosphere.