Affinity-based CE Studies to Facilitate Bioprobe Design and Microbe Detection.

化学系的分析和表面化学项目支持维克森林大学的Christa Colyer教授和她的团队开发基于毛细管电泳法的快速和灵敏的分析方法，并使用合理设计的生物探针用于蛋白质靶标。应用包括基于CE亲和力定量评估新型方酸和三唑基香豆素染料对蛋白质的非共价结合性能，以及与固相微萃取方法(与日本大阪府大学的Hiroyuki Nakazumi博士、S3I，LLC的David Silcott博士和加拿大滑铁卢大学的Janusz Pawliszyn博士合作)的比较。亲和力CE在配基-受体相互作用中的应用也正在评估中(与约翰·霍普金斯大学的陈立平博士合作)，这些配基-受体相互作用对于参与免疫反应调节的B7家族蛋白质的功能至关重要。使用设计的基于硼酸的生物探针的CE分析正在开发中(与南卡罗来纳大学的Qian Wang博士和Karen Fox博士合作)，以确定对人类健康和安全至关重要的病毒和细菌病原体表面特有的碳水化合物残留物，如流感病毒和炭疽芽胞。改进的细菌分析将支持区分和定量各种完整的蓝藻物种，它们的组成藻胆蛋白，以及它们的四吡咯胆色素。这种独特的三管齐下的CE方法应该会增强对蓝藻在全球碳循环中的作用的理解。在这项研究的同时，Colyer博士参与了各种社区和学术推广项目，例如为High Point大学的本科生举办的暑期研究项目，以及为地区学院和大学的教职员工和学生创建的名为“电泳学工作周末”的研讨会。维克森林大学和日本大阪府大学之间的国际学生交流将使学生为日益跨学科和全球化的科学研究做好更好的准备。此外，还将根据研究结果开发一系列案例研究，为大学教师提供教授现代分离科学和生物传感方法所需的资源。这一系列的案例研究，将被称为“分离短片”，将提交给国家科学基金会资助的国家案例研究教学中心的同行评审集，供发表。

The Analytical and Surface Chemistry Program in the Division of Chemistry supports Prof. Christa Colyer and her group at Wake Forest University to develop rapid and sensitive assays based on capillary electrophoresis (CE) methodologies and employing rationally designed bioprobes for protein targets. Applications include quantitative assessment of the noncovalent binding properties of novel squarylium and triazolylcoumarin dyes towards proteins based on CE affinity, and comparison with solid phase microextraction methods (in collaboration with Dr. Hiroyuki Nakazumi of Osaka Prefecture University in Japan; David Silcott of S3I, LLC; and Dr. Janusz Pawliszyn of the University of Waterloo, Canada). Application of affinity CE to ligand-receptor interactions essential to the function of the B7 family of proteins involved in the regulation of immune responses is also being assessed (in collaboration with Dr. Lieping Chen of Johns Hopkins University). CE-based assays employing designed boronic acid-based bioprobes are being developed (in collaboration with Dr. Qian Wang and Dr. Karen Fox of the University of South Carolina) to identify carbohydrate-based residues unique to the surfaces of viral and bacterial agents of importance to human health and security, such as the influenza virus and Bacillus anthracis (anthrax) spores. Improved bacterial analysis will support differentiation and quantitation of various intact cyanobacteria species, their constituent phycobiliproteins, and their tetrapyrrole bilin pigments. This unique three-pronged CE methodology should enhance understanding of the role of cyanobacteria in the global carbon cycle.In conjunction with this research, Dr. Colyer is engaged in various community and academic outreach ventures, such as a summer research program for undergraduate students at High Point University and the creation of a workshop entitled "Electrophoresis Working Weekend" for the academic enrichment of faculty and students from regional colleges and universities. An international student exchange between Wake Forest University and Osaka Prefecture University in Japan will better prepare students for the increasingly interdisciplinary and globalized nature of scientific research. Additionally, a series of case studies based on research results will be developed to provide college-level instructors with the resources necessary to teach modern separation science and biosensing methods. This series of case studies, to be called "Separation Shorts," will be submitted for publication in the peer-reviewed collection of the NSF-funded National Center for Case Study Teaching in Science.