Regulation and Function of Membrane Lipid Hydrolysis in Lipid-Based Signaling of Plants

细胞膜是产生影响植物生长、发育和胁迫反应的生物活性化合物的丰富来源。本项目研究一类膜脂水解酶磷脂酶D （PLD）及其产物磷脂酸（PA）调控植物存活、生长和生物量积累的分子机制。核心假设是，特定的PLDs和PAs通过与碳代谢和翻译调节中的蛋白质相互作用来调节植物生长。本项目将描述PLD/PA与一组碳代谢酶和另一组翻译调节酶的相互作用。PA/ pld效应相互作用将使用不同的方法进行表征，包括定量生物物理分析和调节复合物中蛋白质和脂质的开放式分析。该研究的智力优势在于发现了基于膜的信号传导与能量代谢和生长控制的直接联系，并确定了植物中具有提高植物产量潜力的新调控过程。这项活动将对科学、教育和社会产生更广泛的影响。这项工作有可能改变目前关于细胞整合胁迫和生长线索以优化植物生产的分子和生化机制的知识。它将提供机会，培养学生和研究人员在新兴的，未开发的学科在植物生物学。它将成为扩大代表性不足群体参与研究的平台。将通过讲座和研讨会、课堂教学、科学会议和出版物传播这些信息，以加强科学和教育。所获得的知识可能适用于开发具有更高抗逆性和生产力的作物植物。

Regulation and Function of Membrane Lipid Hydrolysis in Lipid-Mediated Signaling in PlantsGrant proposal number: IOS-0818740PI: Xuemin WangPROJECT SUMMARY Cell membranes are rich sources for producing bioactive compounds that affect plant growth, development, and stress responses. This project investigates the molecular mechanism by which one class membrane lipid-hydrolyzing enzyme phospholipase D (PLD) and its product phosphatidic acid (PA) mediate plant survival, growth, and biomass accumulation. The central hypothesis is that specific PLDs and PAs mediate plant growth by interacting with proteins in carbon metabolism and translation regulation. This project will characterize the interaction of PLD/PA with one group of enzymes in carbon metabolism and another in translation regulation. The PA/PLD-effector interactions will be characterized using different approaches, including quantitative biophysical analysis and open-ended profiling of proteins and lipids in the regulatory complexes. The intellectual merits of the research are to discover direct connections of membrane-based signaling with energy metabolism and growth control and to identify novel regulatory processes in plants with potential to improve plant production. The proposed activity will have broader impacts on science, education, and society. The proposed work has the potential to transform current knowledge on the molecular and biochemical mechanism by which cells integrate stress and growth cues for optimizing plant production. It will provide opportunities to train students and researchers in emerging, under-explored disciplines in plant biology. It will serve as a platform to broaden participation of underrepresented groups in research. The information will be disseminated to enhance science and education through lectures and seminars, classroom teaching, scientific meetings, and publications. The knowledge gained may be applicable to developing crop plants with enhanced stress tolerance and productivity.