Arabidopsis 2010: The Arabidopsis salicylic acid signaling network: A paradigm for phytohormone signaling

植物激素水杨酸(SA)影响植物生长发育的许多不同过程，并在响应非生物和生物胁迫方面发挥重要作用(S)。其在抗病中的作用(S)得到了最广泛的研究，但仍只有部分了解。为了深入了解SA如何执行其不同的功能，最近通过SA与基质的亲和层析在拟南芥中鉴定了大量可能的SA结合蛋白(PSABP)。研究人员将使用两个强大的高通量筛选，一个拟南芥蛋白微阵列和一个酵母三杂交系统，以确定更多的SABP，以及确认已经确定的pSABP的SA结合活性。这些SABP将利用生化、遗传学、分子、生物信息学和生物物理(X射线结晶学)技术进行表征，以破译它们在生长发育和/或对非生物和生物胁迫的响应中的功能，以及SA结合对这些功能的影响。根据目前的数据和所获得的结果，将构建一个SA信号网络模型(S)，通过对可能的信号或调节模块进行更详细的表征来进行测试，并进行改进以产生SABP的SA信号网络的示范模型，该模型可以作为其他植物激素(和其他小配体)的范例。该项目还将促进两种新的、验证较少的方法的开发，用于大规模鉴定结合小分子(如激素)的蛋白质，用于表征动物和植物中的其他激素或小分子信号网络。此外，植物中确定的SA靶点(SABP)可能在人类中具有对应的作用，这些靶点在调节SA和阿司匹林(ACETSA)的多种治疗特性方面具有重要作用，包括降低发热、疼痛、炎症以及心脏病发作、中风和癌症的风险。该项目将为研究生和博士后提供一个刺激的培训环境，在那里他们将接触到大量的生化、分子、遗传、结构和生物信息学方法，以解决植物生物学中的基本问题。它还将为高中生和本科生提供机会，在博士后研究员、研究助理或研究生的密切指导下，亲身体验发现的兴奋。研究成果将通过同行评议的文章在允许广泛电子获取的期刊上广泛传播。此外，新的SABP的身份(一旦它们的约束力通过两个独立的化验得到核实)及其可能参与的各种工厂进程将在发表在同行评议的期刊上之前，通过一个新的网站提供。

The plant hormone salicylic acid (SA) affects many diverse processes in plant growth and development and plays important role(s) in response to abiotic and biotic stresses. Its role(s) in disease resistance has been the most extensively studied but is still only partially understood. To obtain insights into how SA carries out its varied functions, a large number of putative SA-binding proteins (pSABPs) have recently been identified in Arabidopsis by affinity chromatography using SA attached to a matrix. The investigators will use two powerful high throughput screens, an Arabidopsis protein micro-array and a yeast three hybrid system, to identify additional SABPs, as well as confirm the SA-binding activity of the already identified pSABPs. These SABPs will be characterized using biochemical, genetic, molecular, bioinformatic, and biophysical (X-ray crystallography) techniques to decipher their functions in growth and development and/or responses to abiotic and biotic stresses, and the effects of SA binding on these functions. Based on current data and the results obtained, an SA signaling network model(s) will be constructed, tested by more detailed characterization of putative signaling or regulatory modules, and refined in order to produce an exemplary model of the SA signaling network of SABPs which may serve as a paradigm for other plant hormones (and other small ligands). The project will also facilitate development of the two new, less proven approaches for large scale identification of proteins that bind small molecules (like hormones), for their use in characterization of other hormones or small molecule signaling networks in animals as well as plants. Moreover, the SA targets (SABPs) identified in plants may have counterparts in humans that are important in mediating SA's and aspirin's (aceto SA) diverse therapeutic properties including reduction in fever, pain, inflammation, and risk of heart attack, stroke and cancer. The project will provide a stimulating training environment for graduate and postdoctoral students, where they are exposed to a large repertoire of biochemical, molecular, genetic, structural, and bioinformatic approaches to address fundamental questions in plant biology. It also will provide high school students and undergraduates with opportunities to experience first-hand the excitement of discovery under the close mentorship of a postdoctoral fellow, research associate or graduate student. Research results will be disseminated widely through peer-reviewed articles in journals that allow broad electronic access. Moreover, the identity of new SABPs (once their binding is verified by two independent assays) and their possible involvement in various plant processes will be made available through a new website before publication in peer-reviewed journals.