Advancing cITP-NMR and Reverse-phase Ion-Pairing Separations of Anionic Oligosaccharides

加州大学河滨分校的Cynthia K. Larive教授在化学系分析和表面化学项目的支持下，致力于开发毛细管等速荧光-核磁共振（ctp - nmr）方法，用于分析和确定来自重要生物聚合物（如肝素和硫酸软骨素）的低聚糖的结构。这项工作还旨在推进反相离子配对色谱分离这些分子的方法，包括魔角旋转核磁共振实验，以帮助理解分析物、离子配对试剂和色谱固定相之间分子间相互作用的本质。该研究为生物化学和制药工业提供了潜在的应用，这些工业严重依赖分离方法来纯化和表征化合物。此外，这项工作为学生（包括本科生和代表性不足的群体成员）提供了体验最先进的分析仪器和方法的机会。研究进展将被纳入课堂，在学生的早期教育阶段带来研究的兴奋。这项工作的教育方面将通过分析科学数字图书馆http://www.asdlib.org进行传播，这是一个免费的门户网站，为分析科学的教师、学习者和实践者提供同行评审和注释的电子资源。

Professor Cynthia K. Larive of the University of California, Riverside is supported by the Analytical and Surface Chemistry Program in the Division of Chemistry to work on the development of capillary isotachophoresis-nuclear magnetic resonance (cITP-NMR) methodology for the analysis and structure determination of oligosaccharides from important biopolymers like heparin and chondroitin sulfate. This work also aims to advance methods for the separation of these molecules by reverse-phase ion-pairing chromatography and includes magic angle spinning NMR experiments as an aid to understanding the nature of the intermolecular interactions among the analyte, the ion pairing reagent, and the chromatographic stationary phase.This research offers potential applications in biochemistry and the pharmaceutical industry, which rely heavily on separation methods for compound purification and characterization. In addition, the work offers students (including undergraduates and members of underrepresented groups) opportunities for experience with state-of-the-art analytical instrumentation and methodology. Research advances will be incorporated in the classroom, bringing the excitement of research to students at an early stage of their education. Educational aspects of this work will be disseminated through the Analytical Sciences Digital Library, http://www.asdlib.org, a free portal of peer-reviewed and annotated electronic resources for teachers, learners and practitioners of analytical science.