Collaborative Research: Chemometrics and Comprehensive Two-Dimensional Liquid Chromatography: Toward Achieving the Promise for Metabolomics

弗吉尼亚联邦大学(VCU)的萨拉·鲁坦(Sarah Rutan)教授和明尼苏达大学的彼得·卡尔(Peter Carr)教授得到了化学系分析和表面化学计划的支持，以开发二维液相色谱的定量方面，重点是在代谢组学中的应用。具体地说，这项工作涉及分离条件的优化和新方法的探索，以加速第二维分离和结果数据的分析。其目的是将最多是半定量的耗时过程转变为一种快速有效的方法，为生物标记物识别等应用提供准确的定量结果。如果成功，这项工作将为生物科学家提供重要的新工具，并将有助于更好地了解复杂的生物系统。为了使更广泛的受众更容易理解代谢组学的概念，将开发一个网站，其中包括新陈代谢和分离的在线模拟器，以及为高中课堂设计的相关活动。

Professors Sarah Rutan at Virginia Commonwealth University (VCU) and Peter Carr at the University of Minnesota are supported by the Analytical and Surface Chemistry Program in the Division of Chemistry to develop quantitative aspects of two-dimensional liquid chromatography, with a focus on applications in metabolomics. Specifically, this work addresses the optimization of the separation conditions and exploration of novel means to accelerate both the second dimension separation and the analysis of the resulting data. The aim is to transform a time-consuming process that is semi-quantitative at best into a rapid and efficient method that will provide precise quantitative results for applications such as biomarker identification. If successful, the work will provide important new tools for biological scientists and will contribute towards improved understanding of complex biological systems. In order to make the concepts of metabolomics more accessible to a broader audience, a website with online simulators for metabolism and separations will be developed, along with associated activities designed for use in the high school classroom.