SBIR Phase II: Hybridization and SNP Detection Using Unlabeled Target DNA

该奖项是根据2009年美国复苏和再投资法案（公法111-5）资助的。该SBIR第二阶段项目将提供在任何地点进行远程核酸检测（NAT）的技术。（a）我们的探针-靶标-报告基因（PTR）检测试剂盒（可检测未标记的靶标DNA）、（B）我们的Parallume光学编码技术（可在每次检测中多重检测大量样本）和（c）基于500美元商用CMOS（完全便携）的廉价电池供电成像系统的组合将用于构建自主NAT平台。该系统将用于检测和防御即将入侵的加州柑橘作物的黄龙病菌及其昆虫载体/宿主，导致100%致命的和不可治愈的柑橘绿化病的柑橘。合作者Isca Technologies将提供一种“前端”仪器，该仪器可以通过测量昆虫飞过激光幕时的翅膀跳动频率来选择性地识别昆虫。这种选择性昆虫诱捕器将提供主要是所需昆虫载体和病原体的过滤匀浆，其将在现场用我们的PTR进行分析。这些数据将传送到一个中央数据库，该数据库可以提供对昆虫媒介的位置和细菌负荷的真实的时间评估。作为该项目一部分正在开发的核酸检测（NAT）技术代表了在传统实验室或临床环境之外进行检测的能力的重大进步。该技术可用于检测靶DNA序列，而无需化学标记样品，从而允许在任何位置进行NAT分析。该NAT技术可以与我们的Parallume光学编码技术相结合，从而可以同时测量多个样品。在完全便携的系统上同时分析多个DNA而无需访问实验室的能力将允许NAT在低资源设置中执行或用于世界各地的农业应用。

This award is funded under the American Recovery and Reinvestment Act of 2009 Public Law 111-5).This SBIR Phase II project will provide technology to perform remote nucleic acid testing (NAT) in any location. The combination of (a) our Probe-Target-Reporter (PTR) assay which allows the detection of unlabeled Target DNA, (b) our Parallume optical encoding technology which provides the ability to multiplex a large number of samples in each assay and (c) an inexpensive, battery-powered imaging system, based on a $500 commercial CMOS that is completely portable, will be used to build an autonomous NAT platform. This system will be used to detect and defend against the imminent invasion of California's citrus crop by the Liberibacter pathogen and its insect vector/host which causes the 100% fatal and incurable Citrus Greening disease of citrus. Collaborator Isca Technologies will provide a "Front End" instrument which can selectively identify an insect by measuring its wing beat frequency as it flies through a laser curtain. This selective insect trap will provide a filtered homogenate of primarily the desired insect vector and pathogen which will be analyzed with our PTR in the field. The data will be relayed to a central database which can provide a real time assessment of the location and bacterial load of the insect vector.The Nucleic Acid Testing (NAT) technology under development as part of this project represents a substantial advance in the ability to perform assays outside of the traditional laboratory or clinical setting. This technology can be used to detect Target DNA sequences without the need to chemically label the sample thereby allowing the NAT analysis to be performed in any location. This NAT technology can be combined with our Parallume optical encoding technology which allows many sample to be measured simultaneously. The ability to analyze many DNA simultaneously without access to a laboratory on a completely portable system will allow NAT to be performed in Low Resource Settings or for Agricultural applications around the world.