MRI: Acquisition of an Automated Isothermal Titration Microcalorimeter for the Emory Macromolecular Structure and Interactions Suite

这项主要研究仪器奖资助了埃默里大学生物化学系“大分子结构和相互作用套件”的自动小细胞等温滴定微热计的采购。研究小组的长期目标是利用结构生物学和生物物理学技术的结合来了解大分子相互作用的生化基础。新的微热量计增强了埃默里大学的多种研究项目，包括研究核糖体对蛋白质合成的调节；表观遗传学中的蛋白质、DNA和RNA修饰、蛋白质分选、蛋白质降解和抗生素耐药性；脂质运输;以及核受体的功能。了解控制大分子相互作用的生物物理参数为埃默里大学结构生物学项目增加了一个补充维度。因此，新设备通过改善博士后研究员、研究生和本科生获得最先进仪器的机会，加强了生物物理实验方法的培训。此外，高中生和教师将在埃默里大学和佐治亚理工学院的联合暑期项目中获得实践经验，该项目旨在研究嗜极生物核糖体的生物物理特性。这些研究和教学工作的成果将通过摘要和同行评议的出版物以及学生和教员积极参加专业会议而广泛传播。

This Major Research Instrumentation award funds the acquisition of an automated small cell isothermal titration microcalorimeter for the Emory University Department of Biochemistry's "Macromolecular Structure and Interactions Suite." A long-term goal for the assembled team of investigators is to understand the biochemical basis for macromolecular interactions using a combination of structural biology and biophysical techniques. The new microcalorimeter enhances Emory's diverse research programs that include the investigations of protein synthesis regulation by the ribosome; protein, DNA and RNA modifications in epigenetics, protein sorting, protein degradation and antibiotic resistance; lipid transport; and nuclear receptor function. Understanding the biophysical parameters that govern macromolecular interactions adds a complementary dimension to Emory University structural biology programs. The new equipment therefore enhances training in biophysical experimental methods by improving access to state-of-the-art instrumentation for post-doctoral fellows, graduate students and undergraduates. Furthermore, high school students and teachers will get hands-on experience in a joint Emory-Georgia Tech summer program designed to study the biophysical characterization of extremophile ribosomes. The results of these research and teaching efforts will be broadly disseminated through abstracts and peer reviewed publications, as well as by active participation of students and faculty at professional meetings.