Acquisition of a High-Resolution, Accurate Mass, Benchtop LC-MS with Cyber Outreach Capabilities

获得化学研究仪器和设施：多用户(CRIF：MU)计划的这一奖项后，塔尔萨大学的Dale Teters教授及其同事Tyler Johannes、Kenneth Roberts和Kerry Sublette将获得一台基于网络的台式高分辨率、准确质量、液体色谱质谱质谱计(LC-MS/MS)。该奖项将加强各个层面的研究培训和教育，特别是在以下研究领域：(A)DNA中无嘌呤/无嘧啶损伤的确定；(B)利用藻类工程生产生物燃料；(C)人造I基序的表征；(D)蛋白酶体多肽产品的筛选；(E)有机化合物生物降解的中间产物的鉴定；以及(F)选择性碳-碳键形成方法和天然产物的分离。质谱仪(MS)用于通过测量电离后样品中分子成分的质量并通过质谱仪检测来鉴定样品的化学成分。液相色谱是一种先于质谱分析的分离技术。它将混合物分离成几种化学成分，然后由质谱仪进行分析和鉴定。它是发展最快、使用最广泛的分析仪器技术之一。正因为如此，对研究生和本科生进行这项技术的培训是很重要的。除了加强研究项目外，收购LC-MS/MS还将改变几个本科实验室。光谱仪的网络功能将使其能够接触到俄克拉荷马州的许多大学，这些大学拥有大量的美洲原住民和非洲裔美国人，他们在科学和工程领域的代表性不足。

With this award from the Chemistry Research Instrumentation and Facilities: Multi-user (CRIF:MU) program, Professor Dale Teeters and colleagues Tyler Johannes, Kenneth Roberts and Kerry Sublette from University of Tulsa will acquire a cyber-enabled benchtop high-resolution, accurate-mass, liquid-chromatograph mass spectrometer (LC-MS/MS). The award will enhance research training and education at all levels, especially in areas of study such as (a) determination of apurinic/apyrimidinic lesions in DNA, (b) generic engineering of algae to produce biofuels, (c) characterization of artificial i-motifs, (d) screening of proteasomal peptide products, (e) identification of intermediates in the biodegradation of organic compounds, and (f) selective carbon-carbon bond fomation methods and isolation of natural products.Mass spectrometers (MS) are used to identify the chemical composition of a sample by measuring the mass of the molecular constituents in the sample after they are ionized and detected by the mass spectrometer. Liquid chromatography is a separation technique that precedes the mass spectrometry analysis. It separates a mixture into its several constituent chemicals which are then analyzed and identified by the mass spectrometer. It is one of the fastest growing and most widely used analytical instrumentation techniques. Because of this, it is important for graduate and undergraduate students to be trained in the technique. In addition to enhancing research projects, the acquisition of an LC-MS/MS will transform several undergraduate laboratories. The cyber-enabling of the spectrometer will allow outreach to many universities in Oklahoma that have significant Native American and African American populations which are underrepresented in science and engineering.