Increased Depth Penetration in Coherence Imaging Using Multiply Scattered Light

1133222WaxPI建议发展ms/LCI(多次散射/低相干干涉)，以通过具有多次散射光的厚组织进行成像。建议的具体任务是：1.仪器开发：将对系统进行改装，以实现光学扫描和多光谱干涉成像。光学扫描将提高获取2D图像的速度，而包含多个照明波长将允许恢复重要的生化信息，如血红蛋白浓度和氧合。2.理论建模：为了更好地理解这种方法中成像对比度的机制，PI将采取双管齐下的努力来模拟光的传播。给出了一种基于随机游走形式的解析形式，该形式将被用来评估这种成像方式。为了验证这个模型，他们还将使用蒙特卡罗建模以及与实验结果的比较。3.演示厚组织中血红蛋白分布的成像。为了证明该方法的实用性，他们将成像在厚(~1厘米)组织中的血红蛋白分布。样本将包括旨在验证该方法的组织模体，以及体外动物组织，如乳房成像和视网膜成像中将遇到的肌肉和脂肪，这将用于OCT眼科成像，在OCT眼科成像中，脉络膜的穿透仍然是一个挑战。提出的研究将推进一种新的成像方式，具有独特的潜力，以延长成像深度。此外，新的多光谱干涉测量仪器将推动光学工程的发展，而所提出的随机游走模型将促进对光在组织中传播的理解。

1133222WaxThe PI proposes to develop ms/LCI (multiple scattering/low coherence interferometry) to image through thick tissues with multiply scattered light. The specific tasks proposed are: 1. Instrument development:The system will be modified to enable optical scanning and multispectral interferometric imaging. Optical scanning will improve the speed of acquiring 2D images while inclusion of multiple illumination wavelengths will allow recovery of important biochemical information such as hemoglobin concentration and oxygenation. 2. Theoretical modeling:To better understand the mechanisms of imaging contrast in this approach the PI will take a dual pronged effort to model the light propagation. An analytical form is presented which is based on a random walk formalism that will be used to evaluate this imaging modality. To validate this model, they will also use Monte Carlo modeling as well as comparing to experimental results. 3. Demonstrate imaging of hemoglobin distribution in thick tissues.To demonstrate the utility of the method, they will image hemoglobin distribution through thick (~1 cm) tissues. Samples will include tissue phantoms, designed to validate the method, and ex vivo animal tissues, such as muscle and fat as would be encountered in breast imaging and retinal samples as would be used in OCT ophthalmic imaging, where penetration to the choroid has remained a challenge. The proposed research will advance a novel imaging modality with unique potential to extend imaging depth. In addition, new multispectral interferometry instrumentation will advance optical engineering while the proposed random walk model will advance understanding of light propagation in tissues.