Label-Free Electrochemical Capacitance DNA Sensing with Passive Wireless Radio Frequency Identification Technology

在化学学部化学测量和成像项目的支持下，以及刺激竞争研究实验项目办公室（EPSCoR）的共同资助下，波多黎各大学的Carlos R. Cabrera教授、里约热内卢Piedras教授、波多黎各大学马亚圭斯分校的Yi Jia教授及其团队正在开发一种无标签电化学电容DNA传感技术，该技术采用无源无线射频识别传感器技术。DNA固定和杂交过程的检测和表征在许多应用中都很重要，包括环境监测和生物医学传感。目前的DNA检测和鉴定技术往往需要精密的设备和复杂的样品处理。支持的研究重点是利用无源无线集成RFID传感器技术研究DNA电容传感机制和DNA固定和杂交过程中的DNA/表面界面构象表征，旨在开发一种创新的、低成本的芯片实验室DNA传感技术。拟议的低成本、无标签DNA传感技术的发展将使该设备能够在家庭或诊所等护理点用于不同疾病（例如结核病）或有害物质（例如炭疽）的检测。无标签无线射频识别生物传感器的概念验证准备工作将创造可能有益于我们的学生和社会的副产品。除了具体的科学贡献和技术进步将是拟议研究的直接结果之外，这项工作将通过扩大代表性不足的群体在生物传感器技术的重要领域的参与来改善人类社会。该项目将加强波多黎各大学在无线传感器和集成传感技术领域现有的生物传感器研究和教育项目，为本科生和研究生提供研究机会，并提高普遍定期审议的研究竞争力，从而使波多黎各大学受益。该项目还将通过会议、期刊、项目网站和面向K-12学生和教师的开放日，传播新的科学知识、技术创新和教育课程材料，从而对社会产生影响。参加拟议项目的学生将是波多黎各裔，因此所要求的经费将导致攻读科学和工程高级学位和专业职业的西班牙裔和妇女学生人数的增加。

With support from the Chemical Measurement and Imaging Program in the Division of Chemistry, and co-funding from the Office of Experimental Program to Stimulate Competitive Research (EPSCoR), Professors Carlos R. Cabrera at University of Puerto Rico, Rio Piedras, Professor Yi Jia at University of Puerto Rico - Mayaguez and their groups are developing a Label-Free Electrochemical Capacitance DNA Sensing with Passive Wireless Radio Frequency Identification Sensor Technology. Detection and characterization of DNA immobilization and hybridization processes are important in many applications including environmental monitoring and biomedical sensing. Current techniques for DNA detection and characterization often requires sophisticated equipment and complicated sample treatment. The supported research focuses studies on DNA capacitive sensing mechanism and the DNA/surface interfacial conformational characterization during DNA immobilization and hybridization processes with passive wireless integrated RFID sensor technologies, with the aims of developing an innovative, low cost lab-on-a-chip DNA sensing technology.The development of the proposed low-cost, label-free DNA sensing technology will enable the use of the device for different diseases (e.g. tuberculosis) or hazardous materials (e.g. anthrax) detection at point of care, such as at home or clinic. The proof-of-concept preparation of the label-free wireless RFID biosensor will create spin-offs that may benefit our students and society. In addition to the specific scientific contribution and technological advances that will be the direct result of the proposed research, this work will improve human society by broadening the participation of underrepresented groups in an important area of biosensor technology.This project will benefit the University of Puerto Rico by reinforcing existing biosensor research and educational programs in the areas of wireless sensor and integrated sensing technology, provide research opportunities for undergraduate and graduate students, and enhance competitive research capability at UPR. The project will also impact the society by disseminating the new scientific knowledge, technological innovation, and educational course materials through conferences, journals, project website and open house for K-12 students and teachers. The students participating in the proposed project will be of Puerto Rican origin, and thus the requested funding will lead to an increase in the number of Hispanic and women students pursuing advanced degrees and professional careers in science and engineering.