Confinement effects on the conformation, dynamics and phase transitions of biomolecular systems

我们项目的目的是探索几何形状、亲水性和约束条件的柔软性对生物分子和超分子体系的结构、动力学和相行为的影响。我们计划研究几何限制对模型肽弹性蛋白的构象、动力学和“逆相变”的影响，以及淀粉样蛋白肽的自关联和聚集，重点研究淀粉样原纤维的初始成核和生长过程。除了对胰岛淀粉样多肽（IAPP）进行测量外，我们还计划研究约束对聚赖氨酸立体异构体自组装和纤维形成的影响。此外，我们的目标是研究致密蛋白溶液的分子间相互作用和动力学，以及它们在明确定义的纳米孔结构中固-液和液-液分离区域的相行为，包括约束条件的变化（即约束的大小、形式、非均质性和壁的化学性质）。最后，将研究软硬物质约束对脂质中间相结构（拓扑）和相行为的影响。

The aim of our project is to explore the effects of geometry, hydroaffinity and softness of confinement conditions on the structure, dynamics and phase behavior of biomolecular and supramolecular systems. We plan to study the effect of geometrical restrictions on the conformation, dynamics and "inverse phase transition" of the model peptide elastin, as well as the selfassociation and aggregation of amyloidogenic peptides, focusing on the initial nucleation and growth process of amyloid fibrils. In addition to concluding measurements on the islet amyloid polypeptide (IAPP), we are planning to study confinement effects on the self-assembly and fibril formation of polylysine stereoisomers. Moreover, we aim at investigating the intermolecular interactions and dynamics of dense protein solutions as well as their phase behavior in the region of the solid-liquid and liquid-liquid phase separation region in well-defined nanoporous structures, including variations of the confinement conditions (i.e., the size, form, heterogeneity of the confinement, and the chemical nature of the walls). Finally, the effects of soft and hard matter confinement on the structure (topology) and phase behavior of lipid mesophases will be studied.