Nano and Microprinting Equipment for Novel Bioparticle Separations

摘要#1159763P.I.：Adrienne R.Minerick化学和生物分离计划授予的这项NSF奖支持购买和安装BioForce Nano eNablerTM仪器，以促进密歇根理工大学和上半岛地区的生物微粒和蛋白质组分离研究。微型打印工具将使Adrienne Minerick、Caryn Heldt、David Shonnard、Vanjin Jahng等教授的工作成为可能。具体地说，我们将探索高灵敏度、高分辨率的电动蛋白质分离，分子识别血细胞分离，优化纤维素酶催化分离，以及分离视网膜退行性疾病的蛋白质组生物标志物。Nano eNablerTM是生物技术研究社区(BRC)的基本设备基础设施，将被用于为化学和生物分离做出创新贡献。PI提出的所有项目都利用了Nano eNablerTM提供的纳米级精度将生物材料打印到指定位置的多功能、毫微升打印。生物制品分离的潜在应用范围从血红蛋白变异诊断、利用多肽捕获特殊病毒/病原体、优化纤维素酶活性和分离，到蛋白质组分离，并与荧光或基于量子点的指示剂完全兼容。每种应用的共同优势包括显著减少样品消耗、更高的灵敏度、更低的检测限以及更快和更高的分离效率。这种共享仪器将使PI的研究能够满足科学和工业社区对较小规模生物分离的需求，同时更简单的准备，更简单的操作和多功能性。Nano eNablerTM将转变研究能力，加强研究型研究生和本科生的教育和培训，并增加课程教学。私人投资机构有良好的辅导记录，并致力于从代表性不足的群体中积极招募本科生和研究生参加研究活动。Minerick的表面等电聚焦研究有可能影响蛋白质分离、血红蛋白病诊断，从而在全球范围内影响医疗保健实践，并通过减少化学分析的资源利用来促进可持续发展的理念。PI团队在各自领域的智力贡献方面有着良好的出版记录，获得这一仪器将促进创新的和正在进行的生物分离研究项目。

Abstract#1159763P.I.: Adrienne R. MinerickThis NSF award by the Chemical and Biological Separations program supports the acquisition and installation of a BioForce Nano eNablerTM Instrument to facilitate bioparticle and proteomics separation research at Michigan Tech and in the Upper Peninsula region. The microprinting tool will enable work by Professors Adrienne Minerick, Caryn Heldt, David Shonnard, Wan Jin Jahng, and others. Specifically, we will explore high sensitivity, high-resolution electrokinetic protein separations, molecular recognition blood cell separations, optimizing cellulosic enzyme catalysis for separations, and isolating proteomic biomarkers of retinal degenerative disease. The Nano eNablerTM is essential equipment infrastructure for the Biotechnology Research community (BRC) and will be utilized to make innovative contributions to chemical and biological separations. The PI's proposed projects all harness the versatile, femtoliter printing of biological materials to defined locations with nanoscale accuracy offered by the Nano eNablerTM. The potential bioparticle separations applications range from hemoglobin variant diagnosis, exploiting peptides for specialty viral/pathogen capture, optimizing cellulase activity and separations, to proteome isolations and are fully compatible with fluorescence or quantum dot-based indicators. Common advantages with each application include significantly smaller sample consumption, higher sensitivity, lower detection limits, as well as faster and higher separation efficiencies. This shared instrumentation will enable the PI's research to meet the demand in the scientific and industrial communities for smaller scale bioseparations, with concurrent simpler preparation, simpler operation, and versatility. The Nano eNablerTM will transform research capabilities, enhance research graduate and undergraduate student education and training, and augment course instruction. The PIs have strong mentoring track records and are committed to actively recruiting undergraduate and graduate students from underrepresented groups to participate in research activities. Minerick's surface isoelectric focusing research has the potential to impact protein separations, hemoglobinopathy diagnosis, and thus health care practices on a global scale and contributes to the philosophies of sustainability by decreasing resource utilization for chemical analysis. The PI team has a strong publication record of intellectual contributions in their respective fields and acquisition of this instrument will facilitate innovative and ongoing bioseparations research projects.