MRI: Acquisition of an Illumina HiSeq 2000

新罕布什尔大学（UNH）将获得一个高通量（HTP）测序系统，使新英格兰北部的研究小组能够利用基因组技术探索生态学、进化和生物多样性的基本问题。这项研究将促进基础生物学过程的新跨学科知识的产生，并推动基因组学和生物信息学在传统生物学学科中的作用。该仪器是Illumina HiSeq 2500，既快速又灵活，适用于大量应用。它将支持UNH现有的16个实验室的研究和培训计划，4个新的UNH助理教授，以及8个研究小组在区域本科院校使用基因组学来解决基本的生物学问题：？生物体如何应对环境的变化？用户将受益于这种本地的、管理良好的共享仪器，通过(1)大幅减少样品分析的等待时间，(2)密集的样品管理以控制成本并最大化数据输出，以及(3)全面的现场技术和生物信息学支持。Illumina HiSeq 2500将被安置在UNH哈伯德基因组研究中心（HCGS），这是一个已建立的DNA核心设施，拥有服务驱动的技术，管理和生物信息学团队。HCGS还作为区域生物信息学核心设施，满足整个新英格兰北部科学家的生物信息学和连接需求。HTP测序系统生成的数据的管理、存储和安全将由一个专门的IT团队和UNH研究计算和仪器核心设施的基础设施提供。为了有效地解释和利用这些数据，将有两名全职博士级生物信息学人员专门从事HTP序列分析。此外，HCGS与计算机科学教师和学生广泛合作，促进生物信息学专业知识的跨学科发展。这种团队方法和基础设施将确保测序平台的高效运行，并将允许其被最广泛的研究项目使用。UNH是促进本科生研究经验的国家领导者；该HTP测序系统的预期主要用户在他们自己的研究计划中例证了这一承诺。新的测序系统将为本科生提供这样的研究经验，并为研究生和博士后提供研究机会。为了补充和支持研究活动，将举办一系列讲习班，为学生（和教师）提供HTP测序应用和相关生物信息学工具的培训。此外，该系统产生的数据将纳入参与机构的生物学课程，支持在不同领域采用这些技术。将使用这一工具的研究项目已经有了不同学生和教师参与的良好记录，通过麦克奈尔学者项目、UNH SMART项目和UNH REAP项目吸引学生，并通过与传统黑人学院和大学的合作研究项目建立教师伙伴关系。随着新的HTP测序系统促进了基因组学新领域的探索和信息学应用，以回答更广泛的研究问题，这种参与有望继续下去，并不断增长。

A high throughput (HTP) sequencing system will be acquired by the University of New Hampshire (UNH) to enable Northern New England research groups using genome-enabled technologies to explore basic questions in ecology, evolution, and biodiversity. This research will catalyze the generation of new interdisciplinary knowledge of fundamental biological processes and advance the role of genomics and bioinformatics across traditional biological disciplines. The instrument, an Illumina HiSeq 2500, is both fast and flexible, suited to a large array of applications. It will support the research and training programs of 16 existing laboratories at UNH, four new UNH Assistant Professors, and 8 research groups at regional undergraduate institutions using genomics to address the basic biological question: ?How do organisms respond to changes in their environment?? Users will benefit from this local, well-managed, shared instrument through (1) dramatic reductions in wait times for sample analyses, (2) intensive sample management to contain costs and maximize data output, and (3) comprehensive onsite technical and bioinformatics support. The Illumina HiSeq 2500 will be housed in the UNH Hubbard Center for Genome Studies (HCGS), an established DNA core facility with a service-driven technical, management, and bioinformatics team. The HCGS also serves as a regional bioinformatics core facility, meeting the bioinformatics and connectivity needs of scientists throughout northern New England. Management, storage, and security of the data generated by the HTP sequencing system will be provided by a dedicated IT team and infrastructure in the UNH Research Computing and Instrumentation Core Facility. To interpret and utilize the data effectively, two full-time Ph.D.-level bioinformatics staff will be dedicated to HTP sequence analysis. In addition, the HCGS works extensively with computer science faculty and students to promote the cross-disciplinary development of bioinformatics expertise. This team approach and infrastructure will ensure the efficient operation of the sequencing platform and will allow its use by the broadest number of research programs.UNH is a national leader in promoting research experiences for undergraduates; the anticipated major users of this HTP sequencing system exemplify this commitment in their own research programs. The new sequencing system will support such research experiences for undergraduate students, and also provide research opportunities for graduate students and postdoctoral fellows. To complement and support the research activities, a series of workshops will be held to provide training for students (and faculty) in HTP sequencing applications and the associated bioinformatics tools. In addition, data generated by this system will be integrated into courses in the biology curricula of participating institutions, supporting the adoption of these technologies in diverse fields. The research programs that will use this instrument already have a strong record of participation by diverse students and faculty, attracting students through programs such as the McNair Scholars, UNH Project SMART, and UNH REAP, and building faculty partnerships through collaborative research projects with historically black colleges and universities. This participation is expected to continue, and grow, as the new HTP sequencing system facilitates exploration of new areas of genomics and the application of informatics to answering a wider range of research questions.