M3SI - Molecules and Metals for Mass Spectrometric Imaging

所要求的仪器将是利用该小组成员在光学光谱学和质谱学方面的联合专门知识进行成像实验的关键。我们的目标是开发一种组合成像技术，用于生物样品中的金属、小活性物质、低聚物和生物聚合物。使用LA-ICP-MS和成像MALDI MS分析横向分布将用于研究药物对来自几个来源的组织的影响。与柏林、汉堡和埃森的医学院合作，将提供有关药物、纳米粒作为药物输送载体和模型化合物、金属和目标生物聚合物的命运的标本和提出的问题。这将与光学显微光谱成像，特别是荧光、拉曼和红外显微镜相结合，以提供方法的真正联用。将应用和开发分子分析方法，特别是利用高分辨率的MALDI MS在横向和质谱学方面分析改性生物聚合物。

The requested instrumentation will be pivotal for imaging experiments employing the joint expertise of members of the group in optical spectroscopy and mass spectrometry. We aim at the development of a combination of imaging techniques for metals, small active substances, oligomers and biopolymers in biological specimens. Analysis of lateral distributions using LA-ICP-MS and Imaging MALDI MS will be used to study the impact of drug on tissue from several sources. Cooperation with medical schools in Berlin, Hamburg, and Essen will provide the specimens and the questions asked concerning the fate of drugs, nanoparticles as drug delivery vehicles and model compounds, of metals and the target biopolymers. This will be combined with optical microspectroscopic imaging, in particular fluorescence, Raman, and infrared microscopy to provide a true hyphenation of the methods. Methods for the analysis of the molecules will be applied and developed especially for the analysis of modified biopolymers using MALDI MS with high resolution in lateral and mass spectrometric terms.