Optimizing UV-MALDI-MS, direct IR-LDI-MS, and ToF-SIMS/Laser-SNMS technologies for combined molecular imaging of bioactive lipids and other biomolecules

我们建议联合收割机结合我们在IR-LDI/UV-MALDI质谱、ToF-SIMS/Laser-SNMS技术和脂质组学方面的专业知识，建立一个跨学科、跨院系的质谱生物成像平台。我们共同申请资金，用于最先进的MALDI离子迁移质谱仪和新型SIMS组件，这两种组件目前在明斯特WWU都没有。拟议的仪器将用于（1）方法改进和开发用于UV-/IR-LDI，UV-MALDI MS和ToF-SIMS/Laser-SNMS成像的精细方法，以及（2）用于脂质和其他生物分子的无标记成像的这些技术的组合互补用途。生物医学研究的重点将是研究细胞动力学和疾病中的生物活性脂质。我们将高度利用WWU和医学院提供的优秀研究环境和网络，包括非MS分子成像中心。

We propose to combine our expertise on IR-LDI/UV-MALDI mass spectrometry, ToF-SIMS/Laser- SNMS technologies, and on lipidomics to set up an interdisciplinary, interfaculty mass spectrometry bioimaging platform. We jointly apply for funds for a state-of-the-art MALDI ion mobility mass spectrometer and novel SIMS components, both being currently not available at WWU Münster. The proposed instrumentation will be utilized for (1) method improvement and development of refined methodologies for UV-/IR-LDI, UV-MALDI MS, and ToF-SIMS/Laser-SNMS imaging, and (2) for the combined complementary use of these technologies for label-free imaging of lipids and other biomolecules. The biomedical research focus will be on the study of bioactive lipids in cell dynamics and disease. We will capitalize highly on the excellent research environment and network that is available at WWU and the Medical School including non-MS based molecular imaging centers.