UV&IR MALDI REFLECTION TIME OF FLIGHT MASS SPECTROMETER

紫外线

• 批准号: 6292220
• 负责人: Catherine E. Costello
• 金额: $ 40.17万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-15 至 2002-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6292220
• 关键词: biomedical equipment purchase; matrix assisted laser desorption ionization

A Bruker Apex III reflectron matrix-assisted laser desorption/ionization (MALDI-TOF) mass spectrometer (MS) fitted with ultraviolet (nitrogen, 337 nm) and infrared (Er-YAG, 2.94 um) lasers, pulsed ion extraction and post-source decay (PSD) be purchased to serve the needs of six projects that are carried out by a group of nine major users based at Boston University (BU) School of Medicine, BU Goldman School of Dental Medicine (both at the BU Medical Center campus) and the Eunice Kennedy Shriver Center for Mental Retardation, Waltham, MA. These NIH-funded investigators are engaged in (1) determining the templates and nature of post-translational protein modifications leading to cardiovascular disease (Cohen), (2) determining the structure/activity relationships of milk oligosaccharides and glycoproteins that have protective activity for newborns and young children (Newburg), (3) studying proteoglycans in the cornea (Nugent, Trinkaus-Randall) (4) identifying proteins, peptides and other components present in pellicle, the first site of attack and defense on the tooth surface (Oppenheim, Troxler), (5) understanding the proteomic consequences of exposure to cigarette smoke (Taccioli), and (6) characterizing deposits and circulating proteins resulting in amyloid diseases that arise from fibrillogenesis of immunoglobin light chain fragments following indolent plasma cell dyscrasia in the bone marrow (AL), of serum amyloid A protein (systemic amyloidosis, AA) and of the serum protein transthyretin in patients having inherited genetic mutations (familial amyloidotic polyneuropathy, FAP or ATTR), or advanced age (senile cardiac amyloidosis, SCA) (Walsh, Skinner) A group of six minor users at BUMC and other nearby institutions also have need for state-of-the-art MALDI-TOF MS analysis to support studies in Alzheimer's Disease (Abraham), transition states and intermediates in enzyme catalysis (Allen), protein N-glycosylation pathways (Kukuruzinska), the thrombin receptor (Kuliopoulos), presentation of non-peptid antigens to T-cells (Moody) and lipids and apolipoproteins related to atherosclerosis (Small). The Reflex III MALDI-TOF M will be housed in the BUSM MS Resource. Priority will be given to the projects described; minor users at BUMC and the B main campus, as well as the large Resource community will be served in the remaining time. The instrument will be operated and maintained by experienced personnel already associated with the laboratory. The Resource has a strong record for collaboration and education and will assure that users of this instrument system are integrated into these programs.

Bruker Apex III反射矩阵辅助激光解吸/电离配备紫外线的（MALDI-TOF）质谱仪（MS）（氮，337 nm）和红外（Er-YAG，2.94 μ m）激光器、脉冲离子提取和源后衰减（PSD），以满足波士顿大学（BU）医学院的九个主要用户小组开展的六个项目的需要，牙科医学的BU高盛学校（都在BU医疗中心校园）和尤尼斯肯尼迪施莱佛中心精神发育迟滞，沃尔瑟姆，马。这些NIH资助的研究人员从事（1）确定导致心血管疾病的翻译后蛋白质修饰的模板和性质（Cohen），（2）确定对新生儿和幼儿具有保护活性的牛奶寡糖和糖蛋白的结构/活性关系（Newburg），（3）研究角膜中的蛋白聚糖（Nugent，Trinkaus-Randall）（4）鉴定存在于牙表膜中的蛋白质、肽和其他组分，牙表膜是牙齿表面上攻击和防御的第一个位点（Oppenheim，Troxler），（5）了解暴露于香烟烟雾的蛋白质组学后果（Taccioli），和（6）表征导致淀粉样蛋白疾病的沉积物和循环蛋白，所述淀粉样蛋白疾病由骨髓（AL）中惰性浆细胞恶液质后免疫球蛋白轻链片段的原纤维形成引起，（系统性淀粉样变性，AA）和具有遗传性基因突变的患者中的血清蛋白甲状腺素运载蛋白（家族性淀粉样多发性神经病，FAP或ATTR），或高龄（老年性心脏淀粉样变性）（沃尔什，斯金纳）在BUMC和其他附近机构的一组六个未成年人用户也需要最先进的MALDI-TOF MS分析，以支持阿尔茨海默病的研究（亚伯拉罕），酶催化中的过渡态和中间体（艾伦）、蛋白质N-糖基化途径（Kukuruzinska）、凝血酶受体（Kuliopoulos）、非肽抗原向T细胞的呈递（Moody）以及与动脉粥样硬化相关的脂质和载脂蛋白（Small）。Reflex III MALDI-TOF M将存放在BUSM MS资源中。优先考虑所描述的项目;在BUMC和B主校区的小用户，以及大型资源社区将在剩余的时间提供服务。仪器将由实验室相关的经验丰富的人员操作和维护。该资源在合作和教育方面有着良好的记录，并将确保该仪器系统的用户融入这些计划。