Plasmon-Enhanced Chiroptical Biosensors

马萨诸塞州大学的Vincent Rotello教授和他的团队得到了化学测量和成像计划以及化学系的大分子，超分子和纳米化学计划以及化学，生物工程，环境和运输系统（CBET）的国际化学合作（ICC）赠款与Graeme Cooke和Malcolm Kadodwala（英国格拉斯哥大学）的研究小组合作，创建基于等离子体增强手性光学传感的新生物传感器。该技术是高度敏感的，并提供了吸附的分析物蛋白质的结构信息。该团队将把这种信息丰富的换能技术应用于基于阵列的传感，以开发能够以快速且可重复的方式检测血清蛋白水平微小变化的高响应传感器。拟议的研究计划具有基础和应用研究目标。从根本上说，研究人员将探索纳米级手性行为，开发新的见解等离子体场与手性系统的相互作用。在应用方面，麻省大学和格拉斯哥大学的研究人员将调整和优化合成系统、自组装和纳米图案化，以创建更高效的传感器系统，该系统利用手性传感器的信息丰富的输出来提供快速有效的生物传感。该项目还将为学生提供出色的培训：小组之间的交流计划将促进研究，汇集计划的互补链，并在实验室之间转移专业知识。进一步努力扩大拟议的研究，以少数民族参与者将通过路易斯斯托克斯联盟少数民族参与马萨诸塞州和SURE REU计划。英国的研究小组得到英国工程和物理科学研究理事会（EPSRC）的支持。 不同的蛋白质与偏振光的相互作用不同。由这些相互作用产生的签名提供了对蛋白质结构的洞察，并允许识别蛋白质。这种技术被称为圆二色性（CD），通常使用大量的蛋白质进行。在这项拟议的研究中，研究人员将使用纳米图案化材料，将CD高效地应用于微量蛋白质。由此产生的技术将应用于蛋白质传感，为创建通过蛋白质水平变化检测疾病的诊断设备提供潜在工具。

Professor Vincent Rotello and his group at University of Massachusetts are supported by the Chemical Measurement and Imaging Program and the Macromolecular, Supramolecular, and Nanochemistry program in the Division of Chemistry and the Nano-Biosensing program in the Division of Chemical, Bioengineering, Environmental and Transport System (CBET) for an International Collaboration in Chemistry (ICC) grant to work with the groups of Graeme Cooke and Malcolm Kadodwala (University of Glasgow, UK) to create new biosensors based on plasmon-enhanced chiroptical sensing. This technique is highly sensitive and provides structural information of adsorbed analyte proteins. The team will apply this information-rich transduction technique to array-based sensing to develop highly responsive sensors capable of detecting minute changes in serum protein levels in a rapid and reproducible fashion. The proposed research program features both fundamental and applied research goals. Fundamentally, the researchers will to be explore nanoscale chiroptical behavior, developing new insights into the interactions of plasmonic fields with chiral systems. On the applied side, teh UMass and UGlasgow researchers will be tuning and optimizing synthetic systems, self-assembly, and nanopatterning to create more efficient sensor systems that employ the information-rich output of the chiroptical sensors to provide rapid and effective biosensing. This project will also provide excellent training for students: exchange programs between the groups will advance the research, draw together the complementary strands of the program, and transfer expertise between labs. Further efforts to extend the proposed research to minority participants will be made through the Louis Stokes Alliances for Minority Participation at UMass and the SURE REU program. The United Kingdom groups are supported by UK Engineering and Physical Sciences Research Council (EPSRC). Different proteins interact differently with polarized light. The signature that is generated by these interactions provides both insight into protein structure and allows the protein to be identified. This technique is known as circular dichroism (CD), and is typically performed using large amounts of protein. In this proposed research the researchers will use nanopatterned materials to provide highly efficient application of CD to minute quantities of protein. The resulting technology will be applied to protein sensing, providing a potential tool for creating diagnostic devices for detecting disease through changes in protein level.