CSBR: Living Stocks - The Escherichia Coli Genetic Stock Center

该奖项支持耶鲁大学的大肠杆菌基因储备中心(CGSC)。大肠埃希氏菌是一种重要的模式生物，从遗传学和生物化学的角度来看，它可能是最容易被理解的细胞生物。这种高度的理解使大肠杆菌不仅成为一个重要的研究对象，而且成为研究各种生物的实验室主力，以及生物技术的无价工具。CGSC的使命是充当由研究界创造的转基因大肠杆菌菌株的中央储存库，以便其他研究人员和教育工作者可以随时获得这些菌株。为此，CGSC保留了一批约28,000种经基因定义的大肠杆菌K-12衍生物的培养物。该菌株集合中有超过8,000种不同的突变，在已测序的大肠杆菌K-12基因组中的4,111个确定的基因中，超过90%的基因被证实存在突变。这些文化被分发给美国和全球超过65个国家的研究人员和教育工作者。除了文化的分布，CGSC也是一个信息资源，在网上一个完全可搜索的数据库中提供关于菌株、突变、基因、基因产物和基因图谱的信息。重要的是，CGSC还为那些对在收集中使用大肠杆菌菌株有疑问的研究人员提供专家信息和建议的来源。CGSC将继续维护和扩大其关于菌株基因类型、表型、病史和系谱的公开数据库，这是库存中心独有的。信息系统将升级，以便与其他大肠杆菌网络资源更无缝地整合，包括EcoliWiki项目、Ecogene和EcoCyc。CGSC正在与其他国际培养物收集数据库合作，如StraInfo和世界微生物数据中心(WDCM)，以便这些全球菌株数据库可以为收集的细菌编入索引。在获奖期间，将扩大能力，以分发大型系统菌株集，这对对基因组和蛋白质组研究感兴趣的用户很有用。将为那些需要转基因菌株，但没有能力自己构建它的用户提供菌株构建服务，特别是对那些传统细菌遗传学社区以外的用户。访问菌株收集数据库，包括基因类型、基因功能和图谱位置，以及广泛的参考文献，可在(http://cgsc.biology.yale.edu/).)获得

This award supports the E. coli Genetic Stock Center (CGSC) at Yale University. Escherichia coli is an important model organism and is perhaps the best understood cellular organism from a genetics and biochemistry standpoint. This high degree of understanding has made E. coli not just an important subject of research, but also the laboratory workhorse for studying a broad variety of organisms as well as an invaluable tool for biotechnology. The mission of the CGSC is to act as a central repository for genetically modified strains of E. coli created by the research community, so that they may be readily available to other researchers and educators. To that end, the CGSC maintains a collection of ~28,000 cultures of genetically defined derivatives of E. coli K-12. There are over 8,000 different mutations represented in this strain collection with verified mutations in over 90% of the 4,111 identified genes in the sequenced E. coli K-12 genome. These cultures are distributed to researchers and educators in the US and over 65 countries worldwide. In addition to the distribution of cultures, the CGSC is also an information resource, and provides information about strains, mutations, genes, gene products and genetic maps, in a fully searchable database on the web. Importantly CGSC also acts as a source of expert information and advice for those researchers who have questions about using the E. coli strains in the collection. The CGSC will continue to maintain and expand its publicly available database on strain genotypes, phenotypes, histories and pedigrees, which is unique to the Stock Center. Information systems will be upgraded to more seamlessly integrate with other E. coli web resources, including the EcoliWiki project, EcoGene and EcoCyc. The CGSC is collaborating with other international culture collection databases such as StrainInfo and the World Data Centre for Microorganisms (WDCM) so that the collection can be indexed by these global strain databases. Over the period of this award, capabilities will be expanded to distribute large systematic strain sets, which are useful for users interested in genomic and proteomic research. Strain construction services will be offered for those users who require a genetically modified strain, but lack the capability of constructing it themselves, particularly to those outside of the traditional bacterial genetics community. Access to the strain collection database, including genotypes, gene function and map location, as well as an extensive bibliography are available at (http://cgsc.biology.yale.edu/).